Naslov
Glutathione and glutathione S-transferases as early markers for ovarian carcinomas: case series

Autori
Šprem, Marina ; Babić, Damir ; Abramić, Marija ; Vrhovec, Ivan ; Škrk, Janez ; Miličić, Duško ; Ambriović Ristov, Andreja ; Kalafatić, Držislav ; Osmak, Maja

Izvornik
Croatian Medical Journal (0353-9504) 42 (2001), 6; 393-398

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
carcinoma; glutathione; glutathione S-transferases; ovarian neoplasms; urokinase type plasminogen activator; plasminogen activator inhibitor type 1

Sažetak
Aim. To determine the activity of glutathione S-transferases (GST) and concentrations of glutathione (GSH) , urokinase plasminogen activator (uPA) and urokinase plasminogen activator inhibitor type 1 (PAI-1), and to evaluate their diagnostic and prognostic value and possible correlation with clinical and histopathological prognostic factors for ovarian carcinomas. Methods. The concentrations of GSH, uPA, PAI-1 and activity of GST were analyzed in 35 tissue samples taken from 10 normal ovaries, 10 benign, 10 primary malignant and 5 metastatic ovarian tumors. The GSH levels and GST activity were determined by spectrophotometric methods, and uPA and PA-I concentrations by ELISA commercial kits. Results. Glutathione concentrations were significantly higher in primary malignant (126.3+12.8 nmol/mg protein) and metastatic (160.5+24.3 nmol/mg protein) ovarian tumor specimens than in normal ovarian tissue (48.9+8.1 nmol/mg protein, p < 0.03 for both carcinoma groups) or benign ovarian tumor samples (35.2+5.0 nmol/mg protein, p=0.001). The GST activity was significantly higher in primary malignant (245.8+22.7 nmol/min/mg protein) and metastatic (303.7+48.8 nmol/min/mg protein) ovarian tumor tissues that in benign tumor specimens (105.9+16.2 nmol/min/mg protein, p < 0.004 for both carcinoma groups) or normal ovarian tissue samples (113.2+32.0 nmol/min/mg protein, p < 0.04 for both carcinoma groups). There were no statistical differences in uPA and PAI-1 concentrations between the normal, benign and malignant tumor samples. Concentrations of GSH, uPA and PAI-1, and activity of GST were independent from histolopathogical and clinical prognostic factors. Conclusions. Increased GSH concentration and GST activity found in primary malignant and metastatic ovarian tumor samples were independent of histopathological and clinical prognostic factors, suggesting that they could be early markers for ovarian carcinomas.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

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