Naslov
Effect of subminimal inhibitory concentrations of selected antibiotics on a serum sensitivity, phagocytosis and bacterial morphology of Pseudomonas aeruginosa strains

Autori
Drenjancevic, Domagoj ; Talapko, Jasminka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Workshop/Programme and Abstracts Novel antimicrobial agents and strategies for pathogen control/25-26 July 2014 / Knežević, Petar - Novi Sad : Faculty of Sciences, University of Novi Sad, 2014, 38-38

Skup
Novel antimicrobial agents and strategies for pathogen control

Mjesto i datum
Novi Sad, Srbija, 25.07.2014. - 26.07.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
/antibiotics, serum sensitivity, phagocytosis

Sažetak
The aim of this study was to determine the effect of subminimal inhibitory concentrations (subMICs) of ceftazidime, meropenem, gentamicin, ciprofloxacin and azithromycin on the serum sensitivity, sensitivity to phagocythosis and bacterial morpfhology of P. aeruginosa strains isolated from a variety of isolation sites at different medical wards in University hospital Osijek. From a total of 140 P. aeruginosa strains isolated from different clinical specimens a total of 30 serum- resistant strains were selected for testing the effect of subMICs of selected antibiotics to sensitivity to bactericidal activity of fresh human serum and morphology. Among these 30 strains, eight strains were selected for testing the effect of subMICs selected antibiotics to phagocythosis and bactericidal activity of polomorphonuclear neuthrofils (PMNs). Bacteria were exposed to 1/2, 1/4, 1/8, 1/16, and 1/32 x MIC of each antibiotic tested. Bacterial susceptibility to bactericidal activity of fresh human serum before and after bacterial exposure to subMICs was determined Significant difference in serum sensitivity of the strains was observed after the bacteria were exposed to subMICs of ceftazidime, meropenem, ciprofloxacin and azithromycin (p<0.01), while the exposure to subMICs of gentamicin did not affect significantly the sensitivity of tested strains to the serum bactericidal activity. Comparing the number of serum- resistant strains before and after exposure to subMICs of antibiotics, statistically significant differences were determined (p<0.01) after exposure of the strains to 1/2, 1/4, 1/8, 1/16 and 1/32 x MIC of meropenem, after exposure to 1/2, 1/4, 1/8 and 1/16 x MIC of ceftazidime, 1/2, 1/4 and 1/8 x MIC of ciprofloxacin and after exposure to 1/2 and 1/4 x MIC of azithromycin. Among all tested antimicrobial agents, only subMICs of azithromycin has influenced the sensitivity of the selected strains of P. aeruginosa to the phagocythosis and bactericidal activity of PMNs statistically significant at the concentration of 1/2 x subMIC. SubMICs of ceftazidime, meropenem and ciprofloxacin affected not only the sensitivity to serum bactericidal activity of bacteria, but also their morphology. The alterations in bacterial morphology caused by subMICs of selected antimicrobial agents could be connected with consecutive bacterial serum sensitivity.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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