Naslov
Pathogenic mechanisms of sepsis in splenectomised mice

Autori
Luetić, Tomislav ; Kovač, Zdenko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
... : Meeting Abstracts ; u: Shock 23 (2005) (S2) / - , 2005, 74-74

Mjesto i datum
,

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
spleen; sepsis

Sažetak
We explored a kinetics of sepsis survival rate and effects of the host modifications on disease parameters in mouse in vivo model. Several aspects of the host reactivity were investigated. Standardized- pooled fecal inoculum when injected intraperitoneally causes strong polymicrobial peritonitis, which progresses to sepsis, shock and eventually leads to death.Fecal inoculum caused a dose dependant sepsis and deaths both in normosplenic and splenectomised mice. We found a statistically significant decrease in survival time of splenectomised animals. The spread of microorganisms throughout the body has an identical pattern in both groups as demonstrated by the microorganism's growth in the tissue samples. The survival rate of splenectomised animals treated with sterile fecal inoculum is 45% lower in comparison to normosplenic animals with the survival rate of 72%. There is a decrease in mortality of septic asplenic animals treated with dexamethasone, while normosplenic animals show a low increase in average survival time. Inhibiton of inducible NO synthase(iNOS) by aminoguanidin increased survival time in splenectomised animals. Overall survival periods as well as severity scores of illness, show identical kinetics in the septic normosplenic and splenectomised TNF-alfa-p55 receptor deficient mice. While septic splenectomised animals showed accelerated mortality kinetics and increased disease severity there were no alterations of tissues bacterial colonisation. Peritonitis induced by sterile inoculum showed better survival rates. Normosplenic animals had no bacterial spread, whereas in splenectomised animals the remote tissues were colonized by bacteria.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

Napomena
Meeting Abstract: 200.

POVEZANOST RADA