Naslov
HLA allele and haplotype polymorphisms among Croatian patients in an unrelated hematopoietic stem cell donor search program

Autori
Grubić, Zorana ; Štingl, Katarina ; Maskalan, Marija ; Serventi-Seiwerth, Ranka ; Mikulić, Mirta ; Burek Kamenarić, Marija ; Nemet, Damir ; Žunec, Renata

Izvornik
Transplant immunology (0966-3274) 31 (2014), 3; 119-124

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Unrelated HSCT ; HLA ; Matching donor ; Searching protocol ; DRB1*11

Sažetak
Aim of the present study was to investigate HLA alleles and haplotypes among Croatian patients in unrelated HSCT program, and to analyze HLA matching in patient/donor pairs. Analysis was performed on a group of 105 patients and their donors, as control served 4000 unrelated donors from our Registry (CBMDR). PCR-SSO and PCR-SSP high-resolution methods for HLA-A, -B, -C, - DRB1, and -DQB1 loci were used for typing patient/donor pairs. Donors from CBMDR were tested for HLA-A, -B, -DRB1 by PCR-SSO. No difference was observed in frequency at HLA tested loci among patients and donors from CBMDR. Fully matched donor (10/10) was found for 68 (64.8%) patients, the highest number of mismatches was found for HLA-DRB1 and HLA-C alleles. The presence of HLA-B alleles (B*15:01, B*18:01, and B*51:01) associated with two or more HLA-C alleles as well as the presence of unusual HLA-B/HLA-C (B*35:01- C*07:01 and B*35:01-C*07:02) combinations resulted in mismatches at HLA-C locus. Additionally, mismatches at DRB1 locus were in most cases found for DRB1*11 alleles. The results suggest that the DRB1*11:04 allele might be considered as a limiting factor in finding a 10/10 matched donor. These data may help in the improvement of the searching protocol for unrelated donors for Croatian patients.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA