Naslov
The effects of hyperbaric oxygenation on the frequencies of B cells, Tcells and Treg in the mesenteric lymph nodes of DSS treated BALB/c mice

Autori
Mihalj, Martina ; Ugor, Emese ; Kellermayer, Zoltan ; Žulj, Marinko ; Pojatić, Đorđe ; Berki, Timea ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / - , 2013

Skup
2nd IMPULSE EFIS-EJI Symposium 2013

Mjesto i datum
Mátraháza, Mađarska, 30.08.2013. - 03.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hyperbaric oxygenation; colitis; dextran sodium sulfate

Sažetak
BECKGROUND AND OBJECTIVES: There is growing body of evidence from studies in both animals and men that hyperbaric oxygenation (HBO2) can ameliorate inflammatory processes in the gut mucosa. Hyperbaric oxygen has been found to reduce gut and serum expression of TNFα and IL-1β, the key mediators involved in inflammatory bowel disease. Furthermore, it has been shown that adoptive transfer or induction of CD4+CD25+FoxP3+ regulatory T cells (Treg) can prevent disease and reverse the course of established colitis. Conversely, two recent studies showed that the transcriptional factor HIF-1α, which is up-regulated by HBO2, can shape the T-cell differentiation towards Th17 (RORγt) over the iTreg (Foxp3) population. The aim of the present study was to investigate the effects of HBO2 on the frequencies of T and B lymphocytes and Treg in mesenteric lymph nodes (mLN) of mice with DSS-induced colitis. For that purpose mice were fed 5% w/v of DSS in drinking water ad libidum for 7 consecutive days and HBO2 was initiated at day 1 and was administered until the end of experiment, twice a day 100% O2 for 60 minutes at 2.3 bars. Disease activity (DAI) was assessed by daily recording of animal body weight, stool consistency and the presence of occult or gross blood per rectum. RESULTS: Starting from day 6 mice treated with DSS presented with severe symptoms of colitis, including weight loss, gross rectal bleeding and diarrhoea. HBO2 significantly reduced disease symptoms, as evidenced by DAI (p≤0.001). Treg frequency in mLN increased significantly in DSS treated mice (27.8±0.67) compared to control mice (11.9±0.67 ; p=0.026) and control mice undergoing HBO2 (11.5±0.65 ; p=0.038). Their frequency was reduced to control values after HBO2 (13.8±0.71 ; p=0.03). Significant increase in frequency of B220+ B cells in mLN of DSS treated mice (from 18.0±1.1% to 47.5±2.35) was followed by relative reduction in CD3+ T-cell frequencies (from 61.2±0.45% to 39.4±1.6%). In addition, they had reduced frequencies of double negative (DN, CD3-B220-) cells. These effects were attenuated by HBO2 (B=30.73±1.6%, T=49.15±1.78). CONCLUSION: DSS induced inflammation of the colon resulted in increased frequencies of Treg cells and B cells in the mLN. Consistent to previous reports HBO2 significantly reduced symptoms of disease and severity of colitis, which was accompanied by reversed frequencies of Treg cells and decreased frequencies of B cells. Our results suggest that the improved course of DSS-induced colitis in BALB/c mice does not involve Treg expansion.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA