Naslov
Quality control and conduct of genome-wide association meta-analyses

Autori
Winkler. T.W. ; .......... Polašek, Ozren ; ... ; Kolčić, Ivana ; ... ; Krželj, Vjekoslav ; ... ; Zgaga, Lina ; ... ; Rudan, Igor ; ... ; Loos, R.J.F.

Izvornik
Nature protocols (1754-2189) 9 (2014); 1192-1212

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
quality control; genome-wide association meta-analyses

Sažetak
Rigorous organization and quality control (QC) are necessary to facilitate successful genome- wide association meta-analyses (GWAMAs) of statistics aggregated across multiple genome- wide association studies. This protocol provides guidelines for (i) organizational aspects of GWAMAs, and for (ii) QC at the study file level, the meta-level across studies and the meta-analysis output level. Real-world examples highlight issues experienced and solutions developed by the GIANT Consortium that has conducted meta-analyses including data from 125 studies comprising more than 330, 000 individuals. We provide a general protocol for conducting GWAMAs and carrying out QC to minimize errors and to guarantee maximum use of the data. We also include details for the use of a powerful and flexible software package called EasyQC. Precise timings will be greatly influenced by consortium size. For consortia of comparable size to the GIANT Consortium, this protocol takes a minimum of about 10 months to complete.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
& The Genetic Investigation of Anthropometric Traits (GIANT) Consortium.

POVEZANOST RADA