Naslov
Chemiluminometric determination of choline-related substances in pharmaceutical preparations by dot- blot
(Chemiluminometric determination of choline-related substances in pharmaceutical preparations by dot- blot)

Autori
Luterotti, Svjetlana ; Maysinger, Dušica

Izvornik
Journal of pharmaceutical and biomedical analysis (0731-7085) 12 (1994), 9; 1083-1090

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Succinylcholine chloride ; oxtriphylline (choline theophyllinate) ; acetylcholine chloride determination ; pharmaceutical preparations ; choline chemiluminometry-dot-blot ; direct standardization

Sažetak
A simple and reliable method of assaying succinylcholine chloride, oxtriphylline (choline theophyllinate) and acetylcholine chloride in pharmaceutical preparations, based on conversion to choline, is provided by combination of chemiluminometry of choline and dot-blot technique. Recoveries of 102 and 97% for choline chloride in Quelicin injection and Choledyl 200 tablets matrices, respectively, and of 99% for acetylcholine chloride in Miochol solution matrix could be achieved using simple choline chloride standards in phosphate buffer pH 8.6. Accordingly, matrix-matched standards were found redundant. Favourable results obtained in preparations- matched media including limits of detection of 37- 39 pmol/microliter of choline chloride, together with accuracies of 0-2% and RSDs ranging from 4 to 7% are the further evidence of the suitability of the method.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA