Naslov
Cutaneous expression of calcium/calmodulin- dependent protein kinase II following diabetes induction

Autori
Borić, Matija ; Jeličić Kadić, Antonia ; Puljak, Livia

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Bridges in Life Sciences 9th Annual Scientific Conference

Mjesto i datum
Split, Hrvatska, 27.05.2014. - 01.06.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
diabetes mellitus; CaMKII; skin

Sažetak
Introduction: Diabetes mellitus (DM) is the leading cause of peripheral neuropathy in the Western world. Calcium/calmodulin-dependent protein kinase II (CaMKII) is one of the key intracellular signaling molecules for neuropathy development. We analyzed the expression of total CaMKII (tCaMKII) and its alpha isoform in the foot pad skin of animal models of diabetes type 1 (DM1) and type 2 (DM2). Methods: Male Sprague-Dawley rats were used (n=40). DM1 was induced with intraperitoneal injection of streptozotocin (STZ) and DM2 with a combination of STZ and high-fat diet. Control rats for both DM1and DM2 were injected with pure citrate buffer solution. Two months after induction of diabetes rats were sacrificed ; skin samples from plantar surface of the both hind paws were removed. Immunohistochemistry was performed for detection of tCaMKII and its alpha isoform. For detection of peripheral nerve fibers we used polyclonal antiserum against protein gene product 9.5 (PGP 9.5). Results: The results showed that CaMKII was expressed in the skin of both diabetic models. Total CaMKII was uniformly distributed throughout the epidermis and pCaMKIIα was limited to stratum granulosum. Both tCaMKII and pCaMKIIα were not visible in neurofibers. Two months after induction of diabetes in rats, significant increase in epidermal expression of tCaMKII and pCaMKIIα was observed in DM1 animals compared to controls, but not in DM2 animals. Discussion: CaMKII was previously found in subepithelial and intraepithelial nerve fibers of facial skin, nasal mucosa and palate. This study is the first description of cutaneous CaMKII expression pattern in a diabetic model. CaMKII could play a role in transformation of skin layers and contribute to cutaneous diabetic changes. Conclusion: These results indicate potential role of CaMKII in diabetic neuropathy development. Further research on physiological role of CaMKII in skin and its role in cutaneous diabetic complications should be undertaken in order to elucidate its function in epidermis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA