Naslov
A HPLC determination of ochratoxin A in human urine from region with endemic nephropathy

Autori
Domijan, Ana-Marija ; Miletić-Medved, Marica ; Peraica, Maja ; Fuchs, Radovan ; Lucić, Ana ; Radić, Božica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology Letters / - Amsterdam : Elsevier, 2001, 42-42

Skup
EUROTOX 2001.

Mjesto i datum
Istanbul, Turska, 13.09.2001. - 16.09.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ochratoxin A; mycotoxin; urine; endemic nephropathy

Sažetak
Humans are exposed to ochratoxin A (OTA) by ingestion of contaminated food of plant and animal origin. A number of methods for OTA determination in food and biological samples are available. The advantage of analysis of OTA in urine vs. analysis in plasma, is simple and not invasive sampling. The aim of this study was to find reliable and economically acceptable method for detection of low levels of OTA in urine and to test it on urine of humans naturally exposed to OTA. Method consisted of liquid and solid-phase extraction clean up. OTA was separated on RP C-18 column, and detected with fluorescent detector. The recovery of the method was 83 % (CV < 10 %), precision evaluated as between-day repeatability was < 7%. Detection limit was 0.4 ng OTA/ml, and quantification limit 0.8 ng/ml of urine. The described method was applied on 71 samples of human urine collected in endemic village (Kaniža) in Croatia. The concentration of OTA in urine was the highest (4.3ą5.4 ng OTA/ml of urine ; meanąSD) in the group of healthy relatives of patients with endemic nephropathy (N=31), lower (1.8ą1.9 ng OT/ml) in persons with some signs of endemic nephropathy (N=19) and the lowest (0.9ą1.2 ng OTA/ml) in healthy persons from the same village (N=21).

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA