Naslov
Serum galectin-3 in osteoporosis and osteoarthritis

Autori
Prunk, Mateja ; Dumić, Jerka ; Marc, Janja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of abstracts / - , 2014, P17-P17

Skup
HDBMB2014 "The Interplay of Biomolecules", Congress of the Croatian Society of Biochemistry and Molecular Biology

Mjesto i datum
Zadar, Hrvatska, 24.09.2014. - 27.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
galectin-3; osteoporosis; osteoarthritis

Sažetak
Osteoporosis and osteoarthritis are disorders common in elderly people and closely associated with disability and morbidity. Development and progression of both diseases is caused by the imbalance of the osteoclastogenesis, osteoblastogenesis and cartilage metabolism. Galectin-3, a member of the β-galactoside– binding proteins family, was found to beinvolved in all of these processes. Consequently the aim of our study was to optimise themethod for determination of galectin-3 in serum and to assess if serum concentration of galectin-3 could be used as a biochemical marker in osteoporosis and osteoarthritis diagnosis. Using the optimised ELISA method the concentration of galectin-3 in sera of 131 Slovene participants, 106 women and 25 men was determined. Galectin-3 concentration was significantly higher in women (p = 0.026, N = 131) while association with body mass index (p = 0.119, N = 126) and age (p = 0.990, N = 131) was not statistically significant. In addition, we found no difference in serum galectin-3 concentration between participants with osteoporosis, osteoarthritis and healthy controls (p = 0.277, N = 131). Moreover the concentration did not correlate with bone mineral density, 25-hydroxy-vitamin D concentration or any of the studied biochemical markers related to bone turnover(C-terminal cross-linking telopeptide of type I collagen, receptor activator of nuclear factor kappa-B ligand, osteoprotegerin or parathormone). However we did found an association with dickkopf 1 concentration in the group of all participants (p = 0.012, N = 47) and in the subgroup of healthy participants (p = 0.023, N = 26), but the association was not significant in the subgroup of osteoporotic participants (p = 0.222, N = 21). Based on the obtainedresults we concluded that serum galectin-3 determination is not useful as a biochemical marker of osteoporosis or osteoarthritis diagnosis. Statistically significant association between galectin-3 concentration and dickkopf-1 concentration points to a possible role of galectin-3 in wnt/ β-catenin signalling pathway that could be further evaluated in additional studies.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Farmacija

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