Naslov
Separation and quantification of carnosine and anserine by microchip electrophoresis using C4D detector

Autori
Jozanović, Marija ; Hajduković, Mateja ; Sak-Bosnar, Milan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 23nd Congress of Chemists and Technologists of Macedonia, Book of Abstracts / - Ohrid, 2014, 34-34

ISBN
978-9989-668-99-9

Skup
XXIII Congress Of Chemists and Technologists of Macedonia

Mjesto i datum
Ohrid, Sjeverna Makedonija, 08.10.2014. - 11.10.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dipeptide ; Karnosine ; Anserine ; Microchip electrophoresis ; Capacitively coupled contactless conductivity detector (C4D)
(dipeptide ; carnosine ; anserine ; microchip electrophoresis ; capacitively coupled contactless conductivity detector (C4D))

Sažetak
Carnosine and anserine are naturally occurring muscle dipeptides found in the skeletal muscles and brain of mammals which inhibit the catalysis of lipid oxidation. They appear to exert a multifunctional effect, acting as free radical scavengers, metal chelators and hydrogen donors. These remarkable substances have broad therapeutic area of applications. They are usually determined by HPLC using various detection principles [1, 2]. Microchip electrophoresis (MCE) is a mode of capillary electrophoresis (CE) in which analysis is performed in a miniaturized device containing microchannels.The concept of MCE was first introduced by Harrison et al. [3] and is widely applied for small biological molecule separations, numerous inorganic cations and anions etc. The quantity of sample actually injected into the microchannel of a chip device is typically in the picoliter (pL) range compared to nanoliter (nL) in CE and microliter (µL) range in liquid chromatography. In the investigation presented, the two biologically important histidine based dipeptides, carnosine and anserine were investigated and successfully separated as a sole species and in their mixtures of various compositions. A capacitively coupled contactless conductivity detector (C4D) operating at several hundreds kHz was used for their detection. The influence of the following parameters on the separation ability and efficiency was investigated: injection voltage, injection time, separation voltage, separation time. The background electrolyte and buffer composition were thoroughly studied in order to optimize the separation. The used disposable chip is fabricated out of poly(methyl methacrylate) (PMMA). The linear response range, detection limits and precision for each particular dipeptide investigated, were determined using linear regression both on a pure standard and in a mixture.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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