Naslov
Identification of quantitative trait loci and candidate genes for specific cellular resistance responses against Didymella pinodes in pea

Autori
Carrillo, Estefanía ; Šatović, Zlatko ; Auber, Grégoire ; Boucherot, Karen ; Rubiales, Diego ; Fondevilla, Sara

Izvornik
Plant cell reports (0721-7714) 33 (2014), 7; 1133-1145

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Quantitative trait loci; Didymella pinodes; Pea; Epidermal cell death; Candidate genes; SNP markers; Cellular resistance

Sažetak
Didymella pinodes, causing ascochyta blight, is a major pathogen of the pea crop and is responsible for serious damage and yield losses. Resistance is inherited polygenically and several quantitative trait loci (QTLs) have been already identified. However, the position of these QTLs should be further refined to identify molecular markers more closely linked to the resistance genes. In previous works, resistance was scored visually estimating the final disease symptoms ; in this study, we have conducted a more precise phenotyping of resistance evaluating specific cellular resistance responses at the histological level to perform a more accurate QTL analysis. In addition, P665 × Messire genetic map used to identify the QTLs was improved by adding 117 SNP markers located in genes. This combined approach has allowed the identification, for the first time, of genomic regions controlling cellular mechanisms directly involved in pea resistance to ascochyta blight. Furthermore, the inclusion of the gene-based SNP markers has allowed the identification of candidate genes co-located with QTLs and has provided robust markers for marker-assisted selection.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA