Naslov
Rats with removed thyroid and parathyroid glands in osteoporosis research

Autori
Dumić-Čule, Ivo ; Grgurević, Lovorka ; Vukičević, Slobodan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
5. International Symposium of Clinical and Applied Anatomy, book of abstracts / - , 2013

Skup
5. International Symposium of Clinical and Applied Anatomy

Mjesto i datum
Graz, Austrija, 24.05.2013. - 26.05.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
thyroparathyroidectomy; osteoporosis

Sažetak
Osteoporosis is one of the most common metabolic diseases of bones. The field of research on osteoporosis has grown in recent years and large number of experimental drugs are tested. For better understanding of drug efficacy and molecular mechanism on bone metabolism establishing of appropriate animal model without influence of hormones that affect bone metabolism would be required. Experiments were carried out in male Sprague-Dawley rats, weighting approximately 350-380g. Removal of the thyroid and parathyroid glands (thyroid-parathyroidectomy - TPTx) was done surgically by a ventral approach under general anesthesia. Blood was collected by retroorbital bleed for serum chemistry, hormones and for determination of bone turnover biomarkers by commercially available kits. The microCT 1076 and analysing software used in these experiment were procured from SkyScan (Kontich, Belgium). Using a materials testing system (TA.HD, The Stable Micro Systems, Goldalming, UK) two types of biomechanical tests were performed on the rat femurs. TPTx resulted in a hypocalcemia with significantly lower serum calcium levels as compared to sham operated control rats. As expected, the TPTx surgery increased serum phosphorus level. TPTx further resulted in a decreased C-telopeptide and osteocalcin serum level as compared to intact rats. TPTx animals had progressively decreasing thyroid hormone and PTH serum level. 1, 25-D(3) was at day 7 decreased about 10 times while TSH increased 4 to 5 times due to the lack of T3/T4. The effect of TPTx on the trabecular bone followed by microCT and biomechanical testing, showed a significantly decreased bone volume and the loss of biomechanical strength. We established TPTx rat model and developed conditions for testing the direct influence of several drugs on bone in the absence of calciotropic hormones. After one week rats had a significant trabecular bone loss and they were ready for experimental research.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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