Naslov
Paracrine regulation of distinct trophoblast functions in vitro by placental macrophages

Autori
Červar Živković, Mila ; Blaschitz, A. ; Dohr, G. ; Desoye, Gerbert

Izvornik
Cell and tissue research (0302-766X) 295 (1999), 2; 297-305

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Human chorionic-gonadotropin; Necrosis-factor-alpha; Monoclonal-antibodies; Extracellular-matrix; Cell-adhesion; Differentiation; Cytotrophoblast; Secretion; Release; Tissue

Sažetak
In view of the accumulating evidence for paracrine mechanisms regulating trophoblast function: we tested the hypothesis that placental macrophages affect trophoblast activity in a paracrine fashion. Trophoblast was isolated from 17 term placentas (-IP). One aliquot of cells was further immunopurified (+IP) using an HLA class I antibody. This increased the proportion of trophoblast (+IP >97% ; -IP similar to 70%) as identified by rigorous immunocytochemistry. Most (similar to 70%) non-trophoblast cells in -IP were macrophages. The cells were cultured for 5 days with a daily medium change. In addition, +IP cells from seven placentas were cultured with lipopolysaccharide (LPS)-stimulated or -unstimulated macrophage-conditioned media. The concentrations of lactate, trophoblast-specific hormones, human chorionic gonadotropin-beta (hCG-beta) and human placental lactogen (hPL), of several prostanoids and of endothelin-l and angiotensin II were determined in the culture media. The accumulated amounts of substances released into the culture media, corrected for the greater proportion of trophoblast in +IP cultures, were on average two- to threefold higher (hCG-beta: 18-fold) in +IP than in -IP, with the exception of endothelin-1, 2 (no change), angiotensin II (-70%) and 6-keto-prostaglandin-F-1 alpha (-40%). [H-3]leucine incorporation into the trichloroacetic acid (TCA)-precipitable pool measured on day 5 was twofold higher in +IP than in -IP. Addition of conditioned media reverted these changes. The data demonstrate that placental macrophages in culture affect trophoblast biosynthetic activity in a paracrine fashion. We conclude that macrophages are important regulators of trophoblast activity.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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