Naslov
Monensin residues in the liver of broiler chickens following drug administration

Autori
Bilandžić, Nina ; Božić, Đurđica ; Varenina, Ivana ; Vrbić, Antonija ; Cvetnić, Luka ; Cvetnić, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Scientific and Professional Conference 15th Ružička Days "Today Science- Tomorrow Industry" / Šubarić, Drago - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku ; Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2014, 41-41

ISBN
978-953-7005-34-4

Skup
15th Ružička Days "Today Science-Tomorrow Industry"

Mjesto i datum
Osijek, Hrvatska, 11.09.2014. - 12.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Monensin; Liver; Broiler; LC-MS-MS

Sažetak
In intensive poultry production, anticoccidial drugs are used and authorised as feed additives for poultry for the prevention and treatment of coccidiosis, an infectious disease caused by several species of Eimeria and Isospora protozoa that predominantly affects young animals [1]. Monensin is a polyether carboxylic ionophore coccidiostat agent commonly used on poultry farms and is authorised for use at a concentration of 100-125 mg kg-1 of feed in chickens for fattening with a withdrawal period of 3 days [2]. The electrostatic property of monensin may cause contamination of feed mill production lines after milling medicated feed, because it is impossible to ensure completely contamination-free production [3]. Kinetic studies in various animal species have shown that the highest levels of monensin residues are excreted in the liver [4]. In the European Union, the maximum residue levels (MRL) for monensin in the liver have been set at 8 μg kg−1 [2]. In this study, monensin residues are determined in the liver tissue of broilers (n=25) treated with feed medicated with monensin concentrations of 325 mg kg-1 for 21 days, which is three times the maximum authorised concentration level. The broilers were killed and liver tissue was collected for 13 days after the end of administration. Residues of monensin were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionisation. The validation parameters of the method were determined in accordance with the criteria of Commission Decision 2002/657/EC [5]: LOD, limit of detection 1.70 g kg-1 ; LOQ, limit of quantification 6.73 g kg-1 ; CCα, limit of decision 9.35 g kg-1 ; recovery 99.5%. The maximum concentrations of monensin of 58.7 g kg- 1 were measured on the first day post-treatment. Concentrations dropped below the MRL value (7.05 g kg-1) on the 4th day and below the LOQ value (2.86 g kg-1) on the 5th day post-treatment. On the 7th day after the end of treatment, monensin levels declined below 1 μg kg-1. The results of the study confirmed the rapid excretion of monensin from liver tissue even when it is administered to broilers at concentrations three times higher than the authorised maximum content. [1] European Commission, Off. J. Eur. Commun. L 268 (2003) 29-43. [2] European Food Safety Authority, EFSA J. 2009 (2011) 1. [3] W. Strauch, Feed Tech. 7 (2003) 23-25. [4] European Food Safety Authority, EFSA J. 592 (2008) 1. [5] European Commission, Off. J. Eur. Commun. L 221 (2002) 8-28.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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