Naslov
Aflatoxin M1 concentrations in raw cow’s milk during the winter months of 2014

Autori
Bilandžić, Nina ; Božić, Đurđica ; Sedak, Marija ; Đokić, Maja ; Vrbić, Antonija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Scientific and Professional Conference 15th Ružička Days "Today Science- Tomorrow Industry" / Šubarić, Drago - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku ; Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2014, 42-42

ISBN
978-953-7005-34-4

Skup
15th Ružička Days "Today Science-Tomorrow Industry"

Mjesto i datum
Osijek, Hrvatska, 11.09.2014. - 12.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Aflatoxin M1; Milk; Winter 2014; ELISA
(Aflatoksin M1; Mlijeko; Zima 2014; ELISA)

Sažetak
Mycotoxin aflatoxins produced by mould species of the genus Aspergillus are known as harmful mutagenic, teratogenic, carcinogenic and immunosuppressive agents [1]. The presence of aflatoxin B1 in the feed of lactating animals leads to the contamination of milk with the hydroxyl-metabolite aflatoxin M1 (AFM1). AFM1 is a stable molecule in raw and processed milk and is unaffected by the process of pasteurisation or treatments used during cheese production [2]. As far as temperature and moisture are concerned, climatic conditions in tropical and subtropical regions favour the growth of the toxigenic Aspergillus [3]. However, long periods of high temperature and long-lasting drought in summer favour the development of these moulds in feed in other climatic regions [4]. The aim of this study is the assessment of AFM1 concentrations in a total of 427 raw cow’s milk samples collected in January (n=143), February (n=151) and March (n=133) of 2014 from farms around Croatia. AFM1 concentrations were measured using a competitive enzyme immunoassay. The method has been validated in accordance with the criteria of Commission Decision 2002/657/EC and the validation parameters were (ng kg-1): LOD, limit of detection 22.2 ; LOQ, limit of quantification 34.2 ; CCβ, detection capacity 33.0. AFM1 levels in the milk were measured in the following range (ng kg-1): 2.81-211.4. Mean AFM1 levels measured in the three months were (ng kg-1): January 11.5, February 15.6, March 10.5. The highest concentration of 211.4 ng kg-1 was determined in February. AFM1 levels exceeded the LOQ value (34.2 ng kg-1) in only 12 samples out of 427 analysed samples. In a total of 8 milk samples (1.87%), AFM1 concentrations exceeded the European Union maximum residue permitted amount of 50 ng kg-1 [6]. The low incidence of elevated AFM1 in the milk indicates a low incidence of contaminated complementary foods, which were used on only a few farms during the winter months of 2014. Seasonal variations of AFM1 concentrations have been previously confirmed in Croatia, with an increase in concentrations during winter when lactating cows are fed with greater amounts of mixed supplementary feedstuff compared to the summer [5]. [1] International Agency for Research on Cancer, IARC monograph on the evaluation of carcinogenic risk to humans, Lyon, France 2002, pp. 171-175. [2] H.H. Oruc, R. Cibik, E. Yilmaz, O. Kalkanli, Food Addit. Contam. 23 (2006) 190. [3] L.C.A. Picinin, M.M.O.P. Cerqueira, E.A. Vargas, A.M.O. Lana, I.M. Toaldo, M.T. Bordignon- Luiz, Food Contr. 31 (2013) 419. [4] L. Decastelli, J. Lai, M. Gramaglia, A. Monaco, C. Nachtmann, F. Oldano, Food Contr. 18 (2007) 1263. [5] N. Bilandžić, Đ. Božić, M. Đokić, M. Sedak, B. Solomun Kolanović, I. Varenina, S. Tanković, Ž. Cvetnić, Food Contr. 40 (2014) 260. [6] European Commission, Off. J. Eur. Commun. L 264 (2006) 5.

Izvorni jezik
Engleski

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Javno zdravstvo i zdravstvena zaštita

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