Naslov
4-Hydroxynonenal and linoleic acid micelles can modulate permeability of an in vitro blood-brain barrier

Autori
Cindrić, Marina ; Tartaro Bujak, Ivana ; Čipak, Ana ; Milković, Lidija ; Mihaljević, Branka ; Žarković, Neven ; Žarković, Kamelija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Joint Meeting: 7th HNE-Club International Meeting & 5th Health Food Symposium - SAS 2014

Mjesto i datum
Toulouse, Francuska, 08.09.2014. - 10.09.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
blood-brain barrier; hydroxynonenal; linoleic acid; permeability

Sažetak
Blood-brain barrier (BBB) represents the main obstacle to the effective treatment of central nervous system (CNS) diseases. Even though lipid peroxidation end products are recognized as important factors in the development and onset of numerous neurodegenerative diseases, their role still needs to be clarified. In the field of brain tumors, 4-hydroxynonenal (HNE) is the most intensively studied aldehyde. It was proven as a valuable prognostic factor in astrocytomas. Besides, co-expression of HNE and CD133 in brain tumor cells, mesenchymal stroma and blood vessels emphasizes the role of HNE in tumor propagation into higher grade. Accelerated CNS tumor progression can be a consequence of increased BBB integrity, thus being aggravating factor in disease treatment. Equally, controlled increase of BBB permeability would result in easier drug flow to the brain. Hence, HNE and micelles of linoleic acid (a major source of essential arachidonic acid in the brain) were used in order to modulate permeability and integrity of BBB in vitro model. The model comprised rat brain endothelial cells (RbE4 cell line) and astrocytes grown on opposite sides of cell culture insert. Changes in permeability and integrity of BBB model were observed through TEER (trans endothelial electrical resistance) measurement and sodium fluorescein passage. Antioxidative status and lipid peroxidation were also assessed. Lipid profiles of the endothelial cells exposed to different treatments were determined. Results revealed that BBB integrity and permeability can be effectively modulated by HNE and linoleic acid micelles. These treatments caused changes in amounts of HNE-modified proteins present in cells constituting the BBB. Antioxidative status of these cells was also affected due to treatments.

Izvorni jezik
Engleski

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