Naslov
Amino acid separation and quantification using microchip electrophoresis

Autori
Horvat, Marija ; Hajduković, Mateja ; Sak-Bosnar, Milan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
14th Ružička Days: International Scientific and Professional Conference "Today Science - Tomorrow industry" / - , 2012, 42-42

Skup
14th Ružička Days "Today Science - Tomorrow industry"

Mjesto i datum
Vukovar, Hrvatska, 13.09.2012. - 15.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Microchip electrophoresis ; C4D ; Amino acids
(microchip electrophoresis ; C4D ; amino acids)

Sažetak
Microchip electrophoresis (MCE) is a mode of capillary electrophoresis (CE) in which analysis is performed in a miniaturized device containing microchannels. The concept of MCE was first introduced by Harrison et al. [1] and is widely applicated for small biological molecule separations, numerous inorganic cations and anions etc. The quantity of sample actually injected into the microchannel of a chip device is typically in the picoliter (pL) range compared to nanoliter (nL) in CE and microliter (µL) range in liquid chromatography. In the investigated presented, several amino acids of different chemical nature were investigated and successfully separated as a single species and in a mixtures of various compositions. A capacitively coupled contactless conductivity detector (C4D) operating at several hundreds kHz was used for their detection. The influence of the following parameters on the separation ability was investigated: injection voltage, injection time, separation voltage, separation time. The background electrolyte and buffer composition were thoroughly studied in order to optimize the separation. The used disposable chip is fabricated out of poly(methyl methacrylate) (PMMA). The linear response region for each particular amino acid investigated, both single and in a mixture, was determined using linear regression. Detection limits for all the amino acids investigated were below 1 mg/mL. [1] D.J. Harrison, A. Manz, Z. Fan, H. Luedi, H.M. Widmer, Anal. Chem. 64 (1992) 1926.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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