Naslov
Isolation of Amino Acids after High-Risk Bio-Waste Processing by Alkaline Hydrolysis

Autori
Kalambura, Sanja ; Racz, Aleksandar ; Darko, Kiš

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Zbornik radova 3rd International Congress on Environmental Helath / Politecnico do Porto - Porto : Politecnico do Porto, 2014

Skup
3rd International Congress on Environmental Helath

Mjesto i datum
Porto, Portugal, 24.09.2014. - 26.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
alkaline hydrolysis parameters; health risks; slaughterhouse waste; waste management

Sažetak
After the occurrence of bovine spongiform encephalopathy and the ban to feed animals with meat-bone meals, new technologies of slaughterhouse waste processing have been developed. Slaughterhouse waste can be very dangerous and potential risk for animal and human health. Brains and spinal cords are deemed high-risk substances and can be infected with prions ; their treatment is therefore only possible in strictly controlled conditions. One of the methods which can achieve the necessary reduction in health risk is alkaline hydrolysis. Standard alkaline hydrolysis is at 150°C, 3-6 hours, and 4 bars. In this investigations focus was on different alkaline conditions: temperature (135ºC, 150ºC, 153ºC), time (2, 3, 6 hours), and base (NaOH and KOH) due to fact that amino acids are sensitive to variations on temperature, pressure and base media. Analysed amino acid composition of hydrolyzed material at different testing conditions show successful hydrolysis. Highest value of protein, which is generally relatively low, was achieved with NaOH at a temperature of 135ºC and time of 2 hours, whereas in the reaction with KOH the highest obtained value was achieved at 150ºC and 3 hours. A dry substance increasing with time, and the highest value was achieved at the temperature of 150ºC and time 6 hours for both bases (NaOH, and KOH). Most of the amino acids went full racemization during the process ; especially in D-amino acids like aspartic acid, glutamic acid, tryptophan and isoleucine. Lysine shows higher resistance to alkaline medium than other amino acids. Isolation of certain amino acids, like: asparagine, glutamic acid, glycine, alanine, and leucine, is possible because they show higher concentrations in hydrolysates.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti

POVEZANOST RADA