Naslov
Biodiesel production: sunflower oil esterification catalyzed by lipase

Autori
Budžaki, Sandra ; Šalić, Anita ; Zelić, Bruno ; Tišma, Marina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Scientific and Professional Conference 15th Ružička days "Today science - tomorrow industry" / Šubarić, Drago ; Planinić, Mirela ; Ačkar, Đurđica ; Pavleković, Ivana - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku ; Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2014, 13-13

ISBN
978-953-7005-34-4

Skup
XV. Ružičkini dani "Danas znanost - sutra industrija"

Mjesto i datum
Vukovar, Hrvatska, 11.09.2014. - 12.09.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biodiesel; lipase; sunflower oil

Sažetak
Biodiesel is a mixture of alkyl esters of fatty acids originated from biological source. Due to its similarities with petroleum diesel, it can be used in diesel engines as alternative fuel. Currently, the industrial production of biodiesel e.g. transesterification is conducted chemically with alkaline catalysts. In this work enzymatic synthesis of biodiesel has been investigated. Enzymatic catalysis offers numerous advantages since it is performed under mild conditions, without additional energy consumptions, with simple glycerol removal and without generation of chemical waste [1]. Transesterifications of edible sunflower oil catalyzed by lipase from Thermomyces lanuginosus (Lipolase 100L) were conducted in laboratory batch and fed-batch reactors using methanol as alkyl donor. Experiments were conducted at three different temperatures (T = 40, 50 and 60 °C) and by two different mechanisms: one-step and four-step reactions. The four-step mechanism was carried out in order to avoid inactivation of the enzyme with methanol [2]. In one-step experiment the molar ratio of oil to methanol was 1 : 3.4, while in the four-step experiment the molar ratio of oil to methanol at the beginning of the process was 1:1 after what methanol was fed into reaction mixture every 12 hours (molar ratio of oil to methanol was 1:0.8). All experiments were performed four days using equal lipase initial concentration ( 0.1 mg/mL ; S.A. = 100 000 U/mg). The highest conversion of fatty acids into corresponding esters of X = 95 % was achieved for one-step reaction performed at 40 °C.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA