Naslov
Modulacija gena stresnog odgovora polifenolima tijekom regeneracije jetre u miša
(Modulation of stress-response genes by polyphenols during liver regeneration in mice)

Autori
Jelena Marinić, Dalibor Broznić, Gordana Čanadi Jurešić, Marin Tota, Čedomila Milin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
15th Ružička days: "Today Science-Tomorrow Industry". Book of abstracts. / Drago Šubarić - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2014, 112-112

ISBN
978-953-7005-31-4

Skup
International Scientific and Professional Conference 15th Ružička days: "Today Science- Tomorrow Industry".

Mjesto i datum
Vukovar, Hrvatska, 11.09.2014. - 12.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
liver regeneration; olive oil polyphenols; hepatic oxidative state; stress sensitive genes

Sažetak
Introduction. Polyphenols interact with nutrient-sensitive transcription factors that coordinate antioxidant response, and thus may be of value in conditions which are challenged by changes in the oxidative state. Therefore, we addressed the ability of the olive oil polyphenols extract (PFE) to induce NF-κB- mediated antioxidant genes expression and concomitant changes in the oxidative state during liver regeneration after 1/3 hepatectomy in mice. Material and Methods.Prior to hepatectomy mice were intraperitoneally administered either PFE (50 mg/kg bw), or saline during seven consecutive days, while respective controls received vehicle alone. The level of serum α- glutathione-S transferase (GST), hepatic malondialdehyde and total glutathione, along with the activities of glutathione reductase (GR) and mRNA levels of NF-κB gene and its targets: γ-glutamylcystein synthetase (GCS), and superoxide dismutase 2 (SOD2) were determined at 0 (controls), 1, 3, 6, 12, 24 and 48 hours after the surgery. Withal, liver mass restoration was calculated. Results. Up to 3 hours after hepatectomy, PFE pre-treatment elevated malondialdehyde, but depleted GSH level and impaired the processes that affect glutathione homeostasis, as indicated by the decreased GR activity, suppressed GSC transcriptional activity, and increased glutathione utilization via the GST mediated reactions. These changes were accompanied by the NF-κB and SOD2 gene upregulation in all time intervals, leading to the normalization of the parameters of oxidative stress and recovery of glutathione status in the periods following 6 hours post- hepatectomy, ultimately resulting in increased liver mass recovery. Conclusion. Increased oxidant load upon polyphenols pre-treatment is associated with induction of NF-κB mediated adaptive stress response which contributes to antioxidant protection and supports the liver growth

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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