Naslov
Two intracellular bacteria in an Amoeba Proteus strain

Autori
Pfannkuchen, Martin ; Schweikert, Michael ; Bruemmer, Franz ; Goertz, H.-D.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
21. Tagung der Deutschen Gesellschaft für Protozoologie im Jahr 2002 / Helmut Plattner - Konstanz : Universitaet Konstanz, 2002, 3-3

Skup
21. Tagung der Deutschen Gesellschaft für Protozoologie im Jahr 2002

Mjesto i datum
Konstanz, Njemačka, 27.02.2002. - 02.03.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Amoeba proteus; endocytobiosis; intracellular bacteria

Sažetak
A strain of Amoeba proteus was isolated from a fresh-water aquarium with tropical fish. The amoebae were identified as A. proteus due to a honey comb structure underneath their nuclear envelope and several other features. Optimal growth of the amebae was observed at 22 C. In the cells two different intracellular bacteria were observed. the bacteria were visible in fixed cells after staining with lacto-aceto orcein or DAPI. One of the bacteria was rod shaped and straight (ro bacteria), whereas the other one was spiral-shaped (sp bacteria). Electron microscopy of thin sectarian revealed a Gram-negative appearance of both bacteria. Ro bacteria appeared to be naked in the host cytoplasm - not encircled by host membranes ; sp bacteria were encircled by two membranes. To get first information about the phylogenetic position of the bacteria fluorescence in situ hybridisation was performed using appropriate oligonucleotide probes. Both bacteria appeared to belong to the Y- subgroup of proteobacteria.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA