Naslov
Direct potentiometric method for human stress determination

Autori
Sakač, Nikola ; Sak-Bosnar, Milan ; Jozanović, Marija ; Regušić, Lidija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
15th Ružička days "Today science - tomorrow industry" BOOK OF ABSTRACTS / Šubarić, Drago - Osijek : Grafika Osijek, 2014

Skup
15th Ružička days "Today science - tomorrow industry"

Mjesto i datum
Vukovar, Hrvatska, 11.09.2014. - 12.09.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
human stress ; ptyalin ; redox sensor

Sažetak
Ptyalin is one of the principal salivary proteins. Ptyalin is a calcium-containing metalloenzyme and degrades starch by hydrolysing its linear α-1, 4-glycosidic linkages, producing dextrins and ultimately maltose [1]. Since ptyalin secretion is regulated by the sympathetic nervous system which stimulates acinar cells of the salivary glands via β-adrenergic receptors, ptyalin activity varies under different conditions. There have been numerous reports on impact of physical exercise on ptyalin activity [2]. All have found a significantly higher concentration of ptyalin during intense exercise in comparison to a control. Exercise is considered as a temporary stress activity. There are several different assay methods available for determination of α-amylase activity. Different industries and laboratories tend to rely on different methods, including spectrophotometry, fluorometry, amperometry, electrophoresis, isoelectric focusing, chromatography and immunological methods [3-5]. Most of the methods are time-consuming and expensive (considering instrumentation, or chemicals). In present, the international standard measuring method of the human ptyalin activity is not assigned, and there is an obvious need to present and register one. In this work, a direct potentiometric method for the determination of ptyalin activity in saliva was proposed. This analytical methodology is based on the direct potentiometric determination of free triiodide ion levels that are reduced due to the amount of remaining nonhydrolysed starch that forms a complex with triiodide [6]. A platinum redox electrode was used as a sensor to detect changes in triiodide concentration, which are directly correlated with ptyalin activity. The used instrumentation is low cost and easy to use, with high microfluidic automatisation potential.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA