Naslov
A closer histological view on microbial life in Mediterranean sponges

Autori
Pfannkuchen, Martin ; Schlesinger, Sabine ; Kloeppel, Anne ; Bruemmer, Franz

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th international sponge symposium. Biodiversity Innovation Sustainability / Custodio, Marcio Reis ; Lobo-Hajdu, Gisele ; Hajdu, Eduardo ; Muricy, Guilherme - Rio de Janeiro : Rio de Janeiro Museu Nacional, 2006, 256-256

ISBN
85-7427-012-1

Skup
7th international sponge symposium

Mjesto i datum
Rio de Janeiro, Brazil, 07.03.2006. - 13.03.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sponge; porifera; microbiology advanced microscopy; histology

Sažetak
Sponges (Porifera) are sessile filter feeders with an enormous filtering capacity and an equally enormous energy consuming throughput resulting in most cases in difficulties in sponge cultivation eg. sufficient nutrient supply. We here show the employment and combination of electron microscopical, light microscopical and molecular biological techniques to approach the function of sponges and their microbial association and their variability during sampling and ex situ cultivation. To have a closer look at sponge associated microbes during cultivation of marine sponges, tissue samples were taken in-situ and during ex situ cultivation. Changes in the bacterial association were correlated to our histological observations and resulting physiology of the sponges eg. secondary metabolites like isofistularin aeroplysinin I and aerophobin I II are measured and do not decrease during ex situ cultivation. Besides transmission electron microscopy (TEM) also paraffin embedding and cryosectioning techniques were applied. In addition, for specific bacterial identification fluorescent in-situ hybridisation (FISH) was used and, for the detection of cyanobacteria their autofluorescence. Additional DAPI staining showed also non autofluorescent bacteria. Transmission electron microscopy identified different bacteria by morphological structures. Combining the data sets resulted, we constructed a three dimensional model of bacterial distribution in sponge tissue and we found a substantially low bacterial load for the in situ condition we investigated. With the different microscopic techniques, especially the optimized fluorescence microscopy, we were able to overcome the main problems like high autofluorescence signal shift and specificity. What is even more, the different methods are controlling themselfes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA