Naslov
Simple and fast voltammetric method for assaying monacolin K in red yeast rice formulated products

Autori
Nigović, Biljana ; Završki, Mario ; Sertić, Miranda ; Mornar, Ana

Izvornik
Food Analytical Methods (1936-9751) 8 (2015), 1; 180-188

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Monacolin K; Lovastatin; Voltammetry; Red yeast rice; Cholesterol-lowering dietary supplements

Sažetak
The red yeast rice formulated products, used worldwide as cholesterol-lowering dietary supplements, have the main active ingredient monakolin K which is actually lovastatin registered as the first statin drug. In this work, a rapid and simple square-wave voltammetric method was developed for determination of monacolin K based on the electrochemical reduction of the carbonyl group in lactone ring. Considering the pH dependence of current response and the interconversion of monacolin K to its acid form, the Britton-Robinson buffer of pH 5.0 was selected as a supporting electrolyte for analytical measurements. The net square-wave voltammetric response of monacolin K was shifted towards more negative potential values by increasing the concentration of analytes. The peak current was linearly proportional to monacolin K concentration in the range of 0.25–20 µg/mL. The fully validated method was successfully applied for fast screening and direct voltammetric determination of monacolin K in red yeast rice products formulated in various dosage forms. To evaluate the proposed voltammetric method the results obtained were compared with those obtained by HPLC methods.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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