Naslov
3-Dimensional reconstruction of fluorescent structures in tardigrades

Autori
Pfannkuchen, Martin ; Brümer, Franz ; Schill, Ralph

Izvornik
Journal of limnology (1129-5767) 66 (2007), 1; 158-163

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
3-dimensional rendering ; ApoTome ; Fluorescence microscopy ; Measuring 3D structures ; Optical sectioning ; Structured illumination ; Tardigrada

Sažetak
Tardigrades are microscopic animals, thus brightfield microscopy is a well established method for tardigrade observation. Modern techniques in functional genetics like fluorescence in situ hybridisation or fluorescently labelled expression markers demand high resolution fluorescence microscopy. Nevertheless tardigrades are still considered to be difficult objects for fluorescence techniques as they are covered by an opaque and diffracting cuticle. We show a modern technique of structured light illumination that enables us to acquire thin optical sections and consequently to reconstruct 3-dimensional structures in tardigrades with a high spatial resolution in all 3 dimensions. This technique is evaluated on taxonomically valuable internal as well as external structures of eutardigrades: the bucco-pharyngeal apparatus and the claws. The 3-dimensional reconstructions allow the measurement of distances in all 3 dimensions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA