Naslov
Effect of initial water concentration and reaction temperature on equilibria of hydrolysis of olive oil catalyzed by Candida rugosa lipase in an emulsion system

Autori
Giacometti, Jasminka ; Milin, Čedomila ; Vasić-Rački, Đurđa ; Giacometti, Fabio

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th European Congress on Biotechnology "Biotechnological Challenges in the new Millennium" : abstracts / Perez Mellado, Rafael - Madrid : Sebiot, 2001, 214-214

Skup
European Congress on Biotechnology "Biotechnological Challenges in the new Millennium" (10 ; 2001)

Mjesto i datum
Madrid, Španjolska, 08.07.2001. - 11.07.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
kinetics of enzyme-catalyzed hydrolysis; Candida rugosa lipase; emulsion system; olive oil

Sažetak
The kinetics of lipase-catalyzed hydrolysis of olive oil by the nonspecific lipase from Candida rugosa has been studied in the emulsion system containing sorbitanmonolaurate as surfactant. The assay of enzyme reaction measurement was carried out in a batch stirred-tank reactor (BSTR) with the pH-stat method. The initial water concentration effect on equilibrium was investigated in the range of 5% to 30% (w/w) water concentrations. The influence of temperature on equilibrium was studied for the range from 27o to 47oC. Since the Michaelis-Menten, equation can not be applied to model the kinetic of enzymatic hydrolysis of olive oil in an emulsion system ; second-order kinetic model was found to be appropriate. The numerical values of the parameters k1, k2 and K were evaluated by fitting the model to the experimental data with the AURORA computer program, which performed the non-linear regression analysis using the Nelder-Mead method. The model was in good agreement with the experimental data for different oil concentrations and different temperatures under reaction conditions in this study. The results show that the high initial water concentration increases equilibrium conversion in the examined range. The influence of temperature on the equilibrium conversion produces the same effect.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija, Prehrambena tehnologija

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