Naslov
Study of developmental stages of microglia in spinal cord explants and organotypic culture

Autori
Sohely Ashraf

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Facolta di scienze mathematiche fisiche e naturali

Mjesto
Trieste

Datum
15.12

Godina
2013

Stranica
66

Mentor
Mladinić Pejatović, Miranda

Ključne riječi
HSP70; AIF; motoneuroni; celastrol; kainična kiselinamicroglia; spinal cord injury; organotypic cultures; in vitro preparation; immunohistochemistry
(microglia; spinal cord injury; organotypic cultures; in vitro preparation; immunohistochemistry)

Sažetak
In vitro preparation of the neonatal rat spinal cord is a practicable tool to investigate the organization of cellular networks and their dysfunction in neurological disease models. Spinal cord organotypic cultures can facilitate our understanding of such physiological and pathological processes over prolonged duration. As microglia mediates the primary immune defense in central nervous system, detailed investigation of their type, number and morphological features in these preparations is necessary to evaluate both the physiological role of microglia and the outcome of experimental damage. The focus of the present immunohistochemical study is the novel characterization of the microglial cell population in the lumbar locomotor region of the postnatal (P0) rat spinal cord at 1–3days in culture, and its comparison with spinal organotypic cultures at 2–5 days in vitro. In freshly isolated neonatal (P0-P3) spinal cord, microglia was found to be 4-6% of the total cell population. After 3 days in culture media, mean percentage of microglia rose to 8% in the white matter and declined to 2% in the grey matter, while retaining the same global number. Such a phenomenon is probably suggestive of a stimulatory effect of serum-containing culture media on microglial cells. On the contrary, in serum free media, global downregulation of microglial population was noted. Unlike the spinal cord case, microglia in organotypic spinal culture showed a dramatic migration towards the peripheral non-neuronal tissue amounting to 8% of the total cell population, while it was only 0.1% in serum free media after 3.5- 5 days. In all in vitro preparations, microglia showed variable shape including amoeboid, round, spindle-like or rod- like instead of the usual ramified pattern observed in vivo. The present report indicates a comparable degree of functional and morphological variations of microglia in spinal cord at developmental age and that this condition is also observed in spinal organotypic cultures.

Izvorni jezik
Engleski

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