Naslov
Humoral immune response in chicken of light line exposed to low dose gamma radiation

Autori
Vilić, Marinko ; Gottstein, Željko ; Pejaković, Jadranka ; Miljanić, Saveta ; Kraljević, Petar

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, The International Congress "Veterinary Science and Profession" / Maltar-Strmečki, N. ; Severin, K. ; Slavica, A. - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2011, 92-92

ISBN
987-953-6062-81-2

Skup
The International Congress "Veterinary Science and Profession"

Mjesto i datum
Zagreb, Hrvatska, 03.10.2011. - 04.10.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Chicken; Gamma radiation; Immune response

Sažetak
In our pervious paper it has been shown that irradiation of commercial meat chicken line eggs by dose of 0.3 Gy before incubation and on the 19th day of incubation increased antibody titre against Newcastle disease virus (NDV) in blood serum. However, to date, little is known of the humoral immunity on the chicken of light line after irradiation by acute low dose radiation. Consequently, the aim of this study was to investigate the antibody response against vaccinal NDV in chicken blood serum after low doses gama irradiation. The chicks of light line were vaccinated against Newcastle disease with a commercial Newcastle disease vaccine (PESTIKAL® La Sota SPF, VETERINA d.o.o., Zagreb, Croatia) by oculonasal route on the 1st day of age. On the 3rd day after vaccination the chicks were irradiated by a dose of 0.05 Gy, 0.15 Gy and 0.8 Gy gamma radiation. Along with the chicks which were irradiated, there were included the same number of chicks unexposed to gamma-radiation. From all chicks blood samples were collected for serological analysis from day 1 up to day 28 in weekly interval. Approximately 1 ml of blood was taken from the right jugular vein. Serum was separated by centrifugation and stored at -18 0C until analyzed. Specific serum anti-NDV antibodies were quantitated by the hemagglutination inhibition (HI) assay as described by OIE. Antibody titre against NDV was significantly increased in the blood serum of chickens irradiated by the dose of 0.05 Gy and 0.3 Gy on the 21st day, while antibody titre against NDV was significantly decreased in the blood serum of chickens irradiated by dose of 0.8 Gy on the 14th day. We presume that such result could be caused by stimulating effect of acute low dose radiation exposure on B-cells and expression of other molecules which participate in simultaneous reactions between cells during immune response. In conclusion we want to emphasize that acute exposure of 0.05 and 0.30 Gy gamma-radiation applied on the 3rd day after vaccination seems to enhance the humoral immune response by stimulating antibody production after vaccination.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA