Naslov
Deconstructing locomotor networks with experimental injury to define their membership

Autori
Nistri, A. ; Taccola, G. ; Mladinić, Miranda ; Margaryan, G. ; Kuzhandaivel, A.

Izvornik
Annals of the New York Academy of Sciences (0077-8923) 1198 (2010); 242-251

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
excitotoxicity ; oxygen glucose deprivation ; reactive oxygen species ; spinal injury ; central pattern generator ; rhythmogenesis

Sažetak
Although spinal injury is a major cause of chronic disability, the mechanisms responsible for the lesion pathophysiology and their dynamic evolution remain poorly understood. Hence, current treatments aimed at blocking damage extension are unsatisfactory. To unravel the acute spinal injury processes, we have developed a model of the neonatal rat spinal cord in vitro subjected to kainate-evoked excitotoxicity or metabolic perturbation (hypoxia, aglycemia, and free oxygen radicals) or their combination. The study outcome is fictive locomotion one day after the lesion and its relation to histological damage. Excitotoxicity always suppresses locomotor network activity and produces large gray matter damage, while network bursting persists supported by average survival of nearly half premotoneurons and motoneurons. Conversely, metabolic perturbation simply depresses locomotor network activity as damage mainly concerns white rather than gray matter. Coapplication of kainate and metabolic perturbation completely eliminates locomotor network activity. These results indicate distinct cellular targets for excitotoxic versus dysmetabolic damage with differential consequences on locomotor pattern formation. Furthermore, these data enable to estimate the minimal network membership compatible with expression of locomotor activity.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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