Naslov
Stress Response and Virulence of Heat-Stressed Campylobacter jejuni

Autori
Klančnik, Anja ; Vučković, Darinka ; Jamnik, Polona ; Abram, Maja ; Smole Možina, Sonja

Izvornik
Microbes and environments (1342-6311) 29 (2014), 4; 338-345

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Campylobacter jejuni ; heat-stress response ; virulence ; food safety

Sažetak
Thermotolerant campylobacters frequently cause bacterial gastroenteritis in humans who are usually infected through consumption of undercooked poultry meat.We examined Campylobacter jejuni heat-stress responses in vitro, after exposure to 48°C and 55°C. Also in vivo modulation of its pathogenicity was investigated, using BALB/c mice intravenously infected with stressed C. jejuni. Regardless of the bacterial growth phase, their culturability and viability in vitro was reduced after exposure to 55°C. This correlated with the altered protein profile and decreased virulence properties seen in vivo. Heat stress at 48°C elicited transition to more resistant bacterial forms, independent of morphological changes or appearance of shorter spiral and coccoid cells. This treatment did not cause marked changes in bacterial virulence properties in vivo. The results indicate that C. jejuni characteristics and pathogenicity in response to heat stress differ depending on temperature. Findings relating C. jejuni response to stresses used during food processing, and modulation of virulence are important to provide a better understanding its contamination and infective cycle and thus contribute to improved safety in food production chain.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Biotehnologija

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