Naslov
Increased perforin expression in multiple sclerosis patients during exacerbation of disease in peripheral blood lymphocytes

Autori
Rubeša, Gordana ; Podack, Eckhard R. ; Sepčić, Juraj ; Rukavina, Daniel

Izvornik
Journal of neuroimmunology (0165-5728) 74 (1997), 1-2; 198-204

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
perforin; multiple sclerosis; peripheral blood lymphocytes; NK; CTL

Sažetak
The expression of perforin (P) in subpopulations of the PBL of multiple sclerosis (MS) patients in stable and active phase of disease was investigated, by simultaneous detection of P (intracellular molecule) and cell surface antigens. A significant increase of CD4+P+ (p<0.02) and CD16+P+ (p<0.001), and decrease of CD56+P+ (p<0.05) cells in active MS was found. In active disease there is highly significant increase (p<0.001) of average fluorescence intensity (AFI) for P in CD4dim+ cells, and these cells are larger in size and have higher granularity (p<0.05) compared to CD4bright+ Pdim+ cells. Surprisingly, there was no CD25+P+ cells in either groups of MS patients. These results show that CD4+P+ cells are upregulated in active disease in cell number, in the level of P expression per cell, and in the level of cell activation (increase in cell size and granularity). It is suggested that CD4+P+ cytotoxic cells may play a role in the pathogenetic mechanisms of MS.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA