Naslov
Determination of aliphatic alcohols, squalene, alpha-tocopherol and sterols in olive oils: direct method involving gas chromatography of the unsaponifiable fraction following silylation

Autori
Giacometti, Jasminka

Izvornik
Analyst (0003-2654) 126 (2001), 4; 472-475

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
gas chromatographic analysis ; sterols ; aliphatic alcohols ; squalene ; tocopherols ; direct method ; silylation ; olive oil

Sažetak
In general, analyses for aliphatic alcohols, sterols, and tocopherols in vegetable oils are performed separately. A simple and reliable procedure is presented for the quantification of the alkanols, squalene, alpha-tocopherol and sterols in olive oils by a direct method involving gas chromatographic (GC) analysis of the unsaponifiable fraction after silylation. The method eliminates the need for a preliminary thin- layer chromatographic (TLC) fractionation prior to GC. External standard calibration with reference substances was used for the quantification of squalene, alpha-tocopherol and sterols, and internal standard calibration for the quantification of aliphatic alcohols. The analyte recovery and the repeatability of the quantitative results were evaluated and were acceptable for routine use.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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