Naslov
Enhanced extraction of isoflavones from Medicago sativa L. by complexation with (2‐hydroxypropyl)‐β‐cyclodextrin and L‐lysine monohydrochloride

Autori
Fumić, Barbara ; Šimić, Sara ; Zovko Končić, Marijana ; Jug, Mario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts HPTLC 2014 / Morlock, Gertrud - Lyon, 2014, 142-142

Skup
International Symposium for High‐Performance Thin‐Layer Chromatography, HPTLC 2014

Mjesto i datum
Lyon, Francuska, 02.07.2014. - 04.07.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Isoflavones; (2 ‐ hydroxypropyl) ‐β‐ cyclodextrin; HPLC

Sažetak
The importance of isoflavones as natural phytoestrogens is currently the subject of intense research and discussions. Phytoestrogens have structural similarity with the human female hormone 17 ‐β‐ estradiol, which can bind to both α‐ and β‐ estrogen receptors, and mimic the action of estrogens on targeted organs, thereby exerting many health benefits when used in some hormone ‐ dependent diseases such as prostate cancer, cardiovascular disease and osteoporosis as well as in relieving postmenopausal symptoms. One of the major difficulties in developing isoflavone ‐ based healthcare products is their low water solubility. Isoflavone aglycones from Medicago sativa L. were isolated in form of ternary complexes with (2 ‐ hydroxypropyl) ‐β‐ cyclodextrin (HP ‐β‐ CD) and L ‐ lysine monohydrochloride, using an ultrasound assisted extraction method. Upon hydrolysis using hydrochloric acid total content of isoflavone aglycones was determined by HPLC. Analysis was run on Agilent 1220 HPLC equipped with variable wavelength detector. Separation was performed on a Agilent Zorbax SB ‐ C18 column, using mobile phases: water/methanol/formic acid (93:5:2) (A) and (3:95:2) (B). Elution was performed at a flow rate of 1 ml/min at the temperature of 40 °C using gradient method. Isoflavone aglycones were identified by the comparison of their retention times with standards acquired commercially. Content of isoflavone aglycones was determined using calibration curves of standards (r 2 > 0.999). Results of the performed HPLC analysis showed that complex formation gained in more efficient extraction of isoflavone aglycones, especially daidzein compared to water extract at HP ‐β‐ CD and L ‐ lysine monohydrochloride concentration of 10 mM. Further studies will examine the influence of higher concentration HP ‐β‐ CD and L ‐ lysine monohydrochloride on isoflavone aglycones extraction.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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