Naslov
Water soluble metalloproteins in Hypogymnia phaisodes

Autori
Falnoga, Ingrid ; Tušek-Žnidarič, Majda ; Jeran, Zvonka ; Jaćimović, Radojko ; Pavičić, Jasenka ; Ivanković, Dušica ; Ščančar, Janez

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Biomonitoring of Atmospheric Pollution (with emphasis on trace elements)-BioMAP II / - Beč : International Atomic Energy Agency (IAEA), 2003, 339-343

Skup
2nd International Workshop on Biomonitoring of Atmospheric Pollution

Mjesto i datum
Praia da Vitória, Portugal, 28.08.2000. - 03.09.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
metal content in lichens; metallothioneins in lichens

Sažetak
The survival of some lichens in environments with elevated levels of metals points to the existence of resistance and tolerance mechanisms in the thalli. In general the major molecules involved in intracellular sequestration and consequent detoxification of metal ions belong to the family of cysteine-rich proteins called metallothioneins. In our investigation the isolation and partial characterisation of such S-containing proteins or polypeptides from the lichen HYPOGYMNIA PHYSODES was attemped. The lichen was taken close to a lead smelter and contained high concentration of lead, zinc and cadmium (263.5, 96.3, 2.8 ug/g). After homogenisation and ultra filtration of the sample the presence of metal binding low molecular weight proteins was identified by SDS electrophoresis and sizeexclusion chromatography (sephadex G-75) where UV absorption and the concentration of metals were determined in column eluents.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA