Naslov
Effect of hyperbaric oxygenation on epoxygenase protein expression in aorta of healthy and diabetic rats

Autori
Kibel, Aleksandar ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ESH-ISH Joint Meeting Hypertension 2014 : book of abstracts ; u: Journal of Hypertension 32 (2014) (S) / - , 2014, E226-e226

Skup
ESH-ISH Joint Meeting Hypertension 2014

Mjesto i datum
Atena, Grčka, 13.06.2014. - 16.06.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hyperbaric oxigenation; epoxygenase; rats; diabetes

Sažetak
OBJECTIVE: In previous studies, we found that hyperbaric oxygenation (HBO) influences vascular reactivity, possibly through upregulation of epoxyeicosatrienoic acids (EETs). EETs, produced by specific epoxygenase enzymes, have a protective role in many conditions including atherosclerosis and hypertension. Impairment of this role of EETs contributes to endothelial dysfunction in hypertension and increase in EETs production attenuates abnormal renal function. Infusion of EETs decreases mean arterial pressure (MAP) in rats. The aim in this study was to evaluate the effect of HBO on epoxygenase protein expression and blood pressure in healthy or streptozocin- induced diabetic male Sprague-Dawley rats. DESIGN AND METHOD: Rats were divided into control and HBO groups. The HBO group was exposed to 100%O2 for 2 hours (with additional 15 minutes for compression/decompression) daily for 4 consecutive days. On the fifth day, anesthetized animals were sacrificed and aorta samples stored at -80ºC. Western blot analysis was performed with specific antibodies for rat epoxygenases (cytochrome P450 isoforms CYP2J3 and CYP2C11). Epoxygenase expression was quantified relatively to ß-actin expression. MAP was measured in separate anesthethized control and HBO animals after insertion of a femoral artery catheter. T-test or Mann Whitney U test was used to compare HBO and control values, depending on distribution normality. RESULTS: Relative CYP2J3/ß-actin expression was similar between the HBO and control group both in healthy (1.488±1.840 [n=11] vs.1.160±0.785 [n=9]) and in diabetic (1.119±0.495 [n=9] vs. 0.945±0.506 [n=11]) rats. Relative CYP2C11/ß-actin expression was similar between healthy HBO and control animals (1.295±1.031 [n=9] vs.1.425±1.174 [n=8]), but it was statistically significantly higher in the HBO group of diabetic rats compared to control (1.395±0.448 [n=7] vs. 0.796±0.534 [n=7] ; P<0.05, t-test). The used HBO protocol did not significantly alter MAP in either healthy (118.9±8.4 HBO [n = 6] vs. 113.0±3.4 mmHg control [n=6]) or diabetic (105.9±4.7 HBO [n=6] vs. 102.3±5.0 mmHg control [n=6]) rats. CONCLUSIONS: The significant upregulation of CYP2C11 protein expression in diabetic rats by HBO suggests that upregulation of EETs production might partially explain beneficial vascular effects of HBO in diabetic subjects. However, direct alteration of MAP with this HBO protocol was not observed.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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