Naslov
Endothelial lipase plasma levels and functional properties of high density lipoprotein in patients with various degrees of coronary artery disease ; Clinical Medical Sciences - Research Proposal

Autori
Bodrožić Džakić, Tomislava ; Degoricija, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
PhD Programme Biomedicine and Health Sciences PhD Day 2014 Abstract Book / Jakopović, Marko ; Lacković, Zdravko - Zagreb : Kerschoffset, 2014, 148-148

ISBN
978-953-6255-60-3

Skup
University of Zagreb School of Medicine PhD Day 2014

Mjesto i datum
Zagreb, Hrvatska, 23.05.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
endothelial lipase; HDL; coronary artery disease

Sažetak
Introduction: Atherosclerotic coronary artery disease is one of the leading causes of morbidity and mortality worlwide.High levels of low-density lipoprotein (LDL), and low levels of high-density lipoprotein (HDL) contribute to initiation and progression of atherosclerosis. Endothelial lipase (EL) is a recently discovered triglyceride lipase which affects the HDL and changes its activity and plasma level. Hypothesis: Concentration and/or activity of EL correlate with different clinical presentations of coronary artery disease. Certain isoforms of EL affect the properties of HDL and patients with various degrees of coronary artery disease have different characteristics of HDL. Aims: To determinate the concentration and enzyme activity of EL and structural and functional characteristics of HDL in patients with acute coronary syndrome (ACS), and in patients with a symptoms and signs of stable angina pectoris (AP) and compare them. To explore if the concentration and/or activity of EL can be used as a biomarker and/or predictor of progression of coronary artery disease. To determine the structural and functional characteristrics of HDL in patients with ACS and stable coronary artery disease and compare them, to determine if levels of EL correlate with structure and function of HDL. Materials and methods: The study includes patients with acute coronary syndrome and patients who have clinical presentation of stable angina pectoris (two groups of patients). We will gather date on patients medical history, physical characteristics, routine laboratory tests, data on electrocardiography, echocardiography, and coronary angiography (Gensini score). Specific laboratory tests include measuring of levels of EL and isolation of HDL. Isolated HDL will be used for testing of the structural and functional characteristics. Structural properties of HDL will be tested by mass spectometry, while the functional characteristics will be tested in vitro in cell culture (human aortic endothelial cells). Expected scientific contribution: To determine if there is any difference in the concentration of EL in patients with ACS and stable angina pectoris, and thus determine whether the level of EL could be used as a biomarker of different stages of coronary artery disease. Poster code: B-9-129.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA