Naslov
Protočna metoda mjerenja antioksidativnog kapaciteta zasnovana na bienzimskom generiranju ABTS•+ i biamperometrijskom detektoru s interdigitaliziranim zlatnim elektrodama
(Flow Injection Analysis of Antioxidants Capacity Based on Bienzymatic Production of ABTS•+ and Biamperometric Detector with Interdigitated Gold Electrodes)

Autori
Milardović, Stjepan ; Kereković, Irena ; Rumenjak, Vlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
XX hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka / Vrsalović Presečki, Ana ; Findrik, Zvjezdana - Zagreb, 2007

Skup
XX hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Zagreb, Hrvatska, 26.02.2007. - 01.03.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
antioxidant; ABTS; biamperometric detector

Sažetak
Development of flow injection analysis (FIA) is reported for determination of total antioxidant capacity of alcoholic beverages (wines and spirits) by bienzymatically produced ABTS+ and electrochemical detector using interdigitated gold electrode (IDE). The method is based on biamperometric measurements using ABTS+│ABTS redox couple. During the analysis, ABTS radical cation was bienzymatically produced by glucose- oxidase and peroxidase separately immobilized in tubular flow-through reactors. Influence of pH and substrate concentration on enzyme activity were tested in order to obtain the most sensitive mode. The effect of flow rate on operation of the bioreactors was also studied. Good comparison of results (R2 = 0.9942) was established for antioxidant capacity of tested samples between proposed FIA methods and reference spectrometric measurements.

Izvorni jezik
Engleski

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