Naslov
The influence of the nucleotide excision-repair system on mutagenesis in Salmonella typhimurium LT2 after exposure to low doses of monofunctional alkylating agents

Autori
Bačun-Družina, Višnja ; Matić, Ivan ; Matijašević, Zdenka ; Alačević, Marija

Izvornik
Mutation research (0027-5107) 264 (1991), 4; 207-212

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
monofunctional alkylating agents ; low doses ; nucleotide excision repair ; Uvr+-dependent mutagenesis

Sažetak
The role of nucleotide excision repair in the mutagenicity of the monofunctional alkylating agents N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), methyl methanesulfonate (MMS), N-ethyl-N'- nitro-N-nitrosoguanidine (ENNG), and N-ethyl-N- nitrosourea (ENU) in Salmonella typhimurium was examined. The mutagenic potential of the mutagenic agents used increased in the following order: MMS less than ENU less than ENNG less than MNNG. The results obtained confirm the involvement of nucleotide excision repair in the removal of mutagenic lesions from the DNA of S. typhimurium cells exposed to high doses of methylating as well as ethylating agents. At the low doses of all the alkylating agents used, the nucleotide excision repair-proficient strain was mutagenized more efficiently than the uvrB mutant. This phenomenon, a consequence of competition between nucleotide excision-repair enzymes and constitutive O6- methylguanine-DNA methyltransferase, is discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA