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Pregled bibliografske jedinice broj: 700174

Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells

Rajkovic, Andreja; Grootaert, Charlotte; Butorac, Ana; Cucu, Tatiana; De Meulenaer, Bruno; Van Camp, John; Bracke, Marc; Uyttendaele, Mieke; Bačun-Družina, Višnja; Cindrić, Mario
Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells // Toxins, 6 (2014), 8; 2270-2290 doi:10.3390/toxins6082270 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 700174 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells

Autori
Rajkovic, Andreja ; Grootaert, Charlotte ; Butorac, Ana ; Cucu, Tatiana ; De Meulenaer, Bruno ; Van Camp, John ; Bracke, Marc ; Uyttendaele, Mieke ; Bačun-Družina, Višnja ; Cindrić, Mario

Izvornik
Toxins (2072-6651) 6 (2014), 8; 2270-2290

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bacillus cereus ; cereulide ; emetic toxin ; doses ; cell ; toxicity ; differentiated Caco-2

Sažetak
Cereulide (CER) intoxication occurs at relatively high doses of 8 µg/kg body weight. Recent research demonstrated a wide prevalence of low concentrations of cereulide in rice and pasta dishes. However, the impact of an exposure to low doses of cereulide was never studied before. In this research, we investigate the effect of low concentrations of cereulide on the behavior of intestinal cells using Caco-2 cell line. The MTT (mitochondrial 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide) and the SRB (Sulforhodamine B) reactions were used to measure the mitochondrial activity and the measurement of cellular protein content, respectively. Both assays showed that differentiated Caco-2 cells were sensitive to low concentrations of CER (in MTT reaction 1 ng/mL after 3 days of treatment ; in SRB reaction 0.125 ng/mL after 3 days of treatment). Cell counts revealed that cells were released from the differentiated monolayer at 0.5 ng/mL of cereulide. Additionally, 0.5 and 2 ng/mL of CER increased lactate presence in the cell culture medium. Proteomic data showed that cereulide at concentration of 1 ng/mL led towards a significant decrease in energy managing and H2O2 detoxification proteins and to an increase in cell death markers. This is amongst first reports to describe the influence of sub-emetic concentrations of cereulide on a differentiated intestinal monolayer model showing that low doses may induce an altered enterocyte metabolism and membrane integrity.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija



POVEZANOST RADA

Projekti:
058-0583444-3466 - Stresom izazvana raznolikost i evolucija mješovitih bakterijskih kultura (Bačun-Družina, Višnja, MZOS ) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Mario Cindrić (autor)

Avatar Url Višnja Bačun-Družina (autor)

Avatar Url Ana Butorac (autor)

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com

Citiraj ovu publikaciju:

Rajkovic, Andreja; Grootaert, Charlotte; Butorac, Ana; Cucu, Tatiana; De Meulenaer, Bruno; Van Camp, John; Bracke, Marc; Uyttendaele, Mieke; Bačun-Družina, Višnja; Cindrić, Mario
Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells // Toxins, 6 (2014), 8; 2270-2290 doi:10.3390/toxins6082270 (međunarodna recenzija, članak, znanstveni)
Rajkovic, A., Grootaert, C., Butorac, A., Cucu, T., De Meulenaer, B., Van Camp, J., Bracke, M., Uyttendaele, M., Bačun-Družina, V. & Cindrić, M. (2014) Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells. Toxins, 6 (8), 2270-2290 doi:10.3390/toxins6082270.
@article{article, author = {Rajkovic, Andreja and Grootaert, Charlotte and Butorac, Ana and Cucu, Tatiana and De Meulenaer, Bruno and Van Camp, John and Bracke, Marc and Uyttendaele, Mieke and Ba\v{c}un-Dru\v{z}ina, Vi\v{s}nja and Cindri\'{c}, Mario}, year = {2014}, pages = {2270-2290}, DOI = {10.3390/toxins6082270}, keywords = {Bacillus cereus, cereulide, emetic toxin, doses, cell, toxicity, differentiated Caco-2}, journal = {Toxins}, doi = {10.3390/toxins6082270}, volume = {6}, number = {8}, issn = {2072-6651}, title = {Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells}, keyword = {Bacillus cereus, cereulide, emetic toxin, doses, cell, toxicity, differentiated Caco-2} }
@article{article, author = {Rajkovic, Andreja and Grootaert, Charlotte and Butorac, Ana and Cucu, Tatiana and De Meulenaer, Bruno and Van Camp, John and Bracke, Marc and Uyttendaele, Mieke and Ba\v{c}un-Dru\v{z}ina, Vi\v{s}nja and Cindri\'{c}, Mario}, year = {2014}, pages = {2270-2290}, DOI = {10.3390/toxins6082270}, keywords = {Bacillus cereus, cereulide, emetic toxin, doses, cell, toxicity, differentiated Caco-2}, journal = {Toxins}, doi = {10.3390/toxins6082270}, volume = {6}, number = {8}, issn = {2072-6651}, title = {Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells}, keyword = {Bacillus cereus, cereulide, emetic toxin, doses, cell, toxicity, differentiated Caco-2} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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