Pregled bibliografske jedinice broj: 700012
Template Switching During Break-Induced Replication Is Promoted by the Mph1 Helicase in Saccharomyces cerevisiae
Template Switching During Break-Induced Replication Is Promoted by the Mph1 Helicase in Saccharomyces cerevisiae // Genetics, 196 (2014), 4; 1017-1028 doi:10.1534/genetics.114.162297 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 700012 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Template Switching During Break-Induced Replication Is Promoted by the Mph1 Helicase in Saccharomyces cerevisiae
Autori
Štafa Anamarija ; Donnianni Roberto Antonio ; Timashev, Leonid A. ; Lam Alicia, F. ; Symington, Lorraine S.
Izvornik
Genetics (0016-6731) 196
(2014), 4;
1017-1028
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
yeast; DNA repair; BIR
Sažetak
Chromosomal double-strand breaks (DSBs) that have only one end with homology to a donor duplex undergo repair by strand invasion followed by replication to the chromosome terminus (break-induced replication, BIR). Using a transformation-based assay system, it was previously shown that BIR could occur by several rounds of strand invasion, DNA synthesis, and dissociation. Here we describe a modification of the transformation-based assay to facilitate detection of switching between donor templates during BIR by genetic selection in diploid yeast. In addition to the expected recovery of template switch products, we found a high frequency of recombination between chromosome homologs during BIR, suggesting transfer of the DSB from the transforming linear DNA to the donor chromosome, initiating secondary recombination events. The frequency of BIR increased in the mph1Δ mutant, but the percentage of template switch events was significantly decreased, revealing an important role for Mph1 in promoting BIR-associated template switching. In addition, we show that the Mus81, Rad1, and Yen1 structure-selective nucleases act redundantly to facilitate BIR.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija
POVEZANOST RADA
Projekti:
058-0580477-2258 - Palindromi u genomima i mehanizmi zamjene gena u kvasca (Krešimir Svetec, Ivan, MZOS ) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
Profili:
Anamarija Štafa
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE