Pregled bibliografske jedinice broj: 69886
Structure and dynamics of myelin sheath in the peripheral nerves of diabetic rats - an ESR study
Structure and dynamics of myelin sheath in the peripheral nerves of diabetic rats - an ESR study // 3rd European Biophysics Congress / Bayley, P.M. ; Devaux, P.F. ; Go, N. ; Janmey, P. ; Parak, F.G. ; Rigler, R. ; Su (ur.).
München: Springer, 2000. (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Structure and dynamics of myelin sheath in the peripheral nerves of diabetic rats - an ESR study
Autori
Žuvić-Butorac, Marta ; Križ, Jasna ; Simonić, Ante ; Schara, Milan
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
3rd European Biophysics Congress
/ Bayley, P.M. ; Devaux, P.F. ; Go, N. ; Janmey, P. ; Parak, F.G. ; Rigler, R. ; Su - München : Springer, 2000
Skup
3rd European Biophysics Congress
Mjesto i datum
München, Njemačka, 09.09.2000. - 13.09.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
fluidity; myelin lipid phase; diabetes; ethidium bromide; osmotic stress; tripsin
Sažetak
The peripheral neuropathy associated with diabetes has characteristical histopathological findings of axonal degeneration, secondary myelin break down and atrophy. In the present study we examine structural integrity of myelin sheath in the peripheral nerves of short-term streptozotocin treated diabetic rats, using ESSR spectroscopy as a tool in determinig the dynamic state and the structure of mylein lipid phase. Experiments were performed on spin labeled sciatic and sural nerves from STZ-treated Hannover-Whitstar rats and age-matched controls. The spectrum analisys employed a numerical simulation model with the set of fitting parameters that in the same time relate the ESR line shape and the structure and dynamics of the pšrobed environment. The simulation considered threee spectral components weighted and summed in the composite spectrum. The comparative analisys of the results ahowed the fraction of the spectral component II to be significantlly increased in the spectra of diabetic rats, indicating the significant increase in overall fluidity of the myelin structure. The origin of fluidity changes was further investigated using: experimental model for demyelinzation (local injection of ethidium bromide in vivo), proteolitic action of tripsin in vitro, and osmotic myelin swelling in vitro. Analisys and comparison of the results suggested a conlusion in terms of pathologically changed biophysical properties of myelin lipid phase, that may represent the first step in development of diabetic neuropathy.
Izvorni jezik
Engleski
Znanstvena područja
Fizika
