Naslov
Hematological assessment of Vardar chub (Squalius vardarensis) from three rivers in the north- eastern Macedonia

Autori
Valić, Damir ; Kapetanović, Damir ; Vardić Smrzlić, Irena ; Jordanova, Maja ; Rebok, Katerina ; Ramani, Sheriban ; Dragun, Zrinka ; Filipović Marijić, Vlatka ; Krasnići, Nesrete ; Uzunov, Risto ; Cvetkovikj, Aleksandar ; Hajrulai-Musliu, Zehra ; Stojanovski, Stojmir ; Nastova, Rodne ; Kostov, Vasil

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International scientific workshop Influence of active mines on freshwater ecosystems / Dragun, Zrinka - Zagreb : Institut Ruđer Bošković, 2014, 25-26

ISBN
978-953-7941-00-0

Skup
Influence of active mines on freshwater ecosystems

Mjesto i datum
Zagreb, Hrvatska, 12.05.2014. - 16.05.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Vardar chub ; hematocrit ; total serum protein ; refractive index ; serum specific gravity

Sažetak
Pollution of surface water presents the major ecological problem in developed industrial world. Various human activities lead to the deterioration of water quality and mining presents one of those impacts. This need for resources has been proven to have extremely negative influence on all biological as well as physico-chemical characteristics of water. Animals that inhabit waters under severe mining activity diversely respond to environmental changes. The changes of hematological parameters in fish can present the consequences of mining influence on the river water. The objective of this study was to determine how mining activity influence hematological parameters in Vardar chub (Squalius vardarensis). Samples from three rivers in the north-eastern Macedonia were obtained: two under mining influence, Zletovska and Kriva River and Bregalnica River as a non-impacted one. Comparison of the values of hematological parameters (hematocrit (HCT), total serum protein (TSP), refractive index (RI) and serum specific gravity (SG)) of Vardar chub captured in three differently polluted rivers showed significant differences. All four parameters had the lowest values in Zletovska River (HCT 30%, TSP 3.6 g/100 mL, RI 1345, SG 1028), which is characterized by the most prominent water contamination with metals. Hematocrit was also reduced in Kriva River (HCT 35%), where metal contamination and poor microbiological quality of the water was found. Hematocrit has the highest value in Bregalnica River (HCT 39.5%), which was selected for sampling as a reference station. Serum proteins, refractive index and specific gravity were elevated in Vardar chub from the Kriva River (TSP 6.75 g/100 mL, RI 1348, SG 1045). Except of the degree of water pollution with metals in three investigated rivers, there were physiological and health differences between examined Vardar chubs. Consequently correlation of hematological parameters and several physiological parameters was analyzed (total weight, condition factor and sex). None of hematological parameters showed a clear correlation with the weight and condition factor. In all three investigated rivers hematocrit values were observed to be somewhat higher in males than in females (about 5% higher median), but the differences were not statistically significant. The other three parameters had higher values in males only in Kriva River, and the differences were statistically significant only for serum proteins (p <0.05). The association between hematological parameters and parasitic infections of Vardar chub was also investigated. Intestinal parasites were found only in Vardar chub from Bregalnica River and comparison between infected (70%) and uninfected (30%) specimens was made. Although the differences were not significant, there was an indication of higher values of serum proteins, the refractive index and specific gravity in uninfected specimens, while hematocrit showed no association with parasitic infection. Although connections between hematological parameters and some physiological and health indicators of Vardar chub were observed, the differences between the investigated rivers were much more pronounced and suggested the possibility of using hematological parameters as indicators of pollution of river water.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija, Veterinarska medicina

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