Naslov
The differences between patients with dual versus isolated bronchial response after allergen challenge

Autori
Stipić-Marković, Asja ; Miculinić, Neven ; Čvorišćec, Branimir ; Ostojić Vedran ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
EAACI Birmingham / NN - Brisel : EAACI, 1999

Skup
EAACI 99, XVIII kongres

Mjesto i datum
Bruxelles, Belgija, 03.07.1999. - 07.07.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Allergic bronchial reaction; Bronchial challenge; early bronchial reaction late bronchial reaction; lymphocytes; eosinophils; CD4; CDB; CD25; CD45RA

Sažetak
The aim o the study was to compare the changes of peripheral blood CD4+,CDB+, CD25+, CD45RR+, lymphocytes and eosinophils, prior to and 48 hours after Der p allergen induced acute asthmatic attack, in groups ,. with early versus dual bronchial reaction. In parallel, the degree of metacholine induced BHR and severity of allergen induced BHR were compared. 42 patients (32 with asthma, 10 with rhinitis; 28 M, 14 F; 15-37 years) with high titers of total IgE and specific IgE's of class +3 or +4 to specific allergen, where included. The allergen was Croatian National Standard (65300 BU/ampoule).Bronchial tests were done by dosimeter method and reaction defined as PD20FEV1. Immunophenotypisation of lymphocytes was performed by flow cytometry (mAb Becton Dickinson). The results showed early bronchial reaction (mean fall in FEV1 28ą18%) in all patients and late reaction in 43% of them (mean fall in PEF 31ą5%). Patients with late allergic reaction (LAR) had severe degree of early reaction (PD20FEV1al 5,01 mg) while those without LAR had mild degree of early reaction (PD20FEV1al 5,01 mg). The difference is statistically significant (p=0,802). 65% of the patients with the LAR also had severe forms of non-specific BHR (PD20FEV1met 0,2 mmol) which is significantly different from the group without LRR (p=0,0007). Opposite to the functional parameters there were no differences immunological parameters: total IgE (p=0,79), specific IgE (p=0,8), CD4(p=0,96 and p=0,72), CD8 (p=0,23 and p=0,22), CD45RA (p=0,93 and p=0,67), CD25 (p=0,14 and p=0,86), eosinophils (p=0,55 and p=0,85) determined prior to and 48 hours after challenge. The results showed that the functional parameters are better markers of shock organ involvement than immunological parameters. Appropriate immunological parameters to detect immune system aberrations as a part of BHR need to be identified.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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