Naslov
Genotyping of Chlamydia trachomatis and Human Papillomavirus in Clinical Specimens from North- Eastern Croatia

Autori
Bošnjak, Zinka ; Perić, Magdalena ; Pavlinić, Dinko ; Džijan, Snježana ; Roksandić-Križan, Ivana ; Vuković, Dubravka ; Ružman, Nataša ; Burazin, Jelena ; Lauc, Gordan ; Antolović-Požgain, Arlen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
20th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) : Abstract book / - , 2010, 749-749

Skup
European Congress of Clinical Microbiology and Infectious Diseases (20 ; 2010)

Mjesto i datum
Beč, Austrija, 10.04.2010. - 13.04.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Chlamydia trachomatis; HPV; sexually transmitted diseases; omp1 genotyping; serotype distribution

Sažetak
Human papillomavirus (HPV) and Chlamydia trachomatis (Ct) are major causes of sexually transmitted diseases. Clinical consequences of high-risk HPV (HR HPV) infection, especially with HPV types HPV 16 and HPV 18, include abnormal Pap test results, low and high-grade cervical intraepithelial neoplasia and cervical cancer, for which Ct may sometimes be a cofactor.The aim of the study was to determine the prevalence of Ct and age-related profiles of HR HPV infections among cases of other sexually transmitted infections in our county. Due to the chronic and “silent” infection of Ct, fast molecular diagnostics and adequate therapy of the infected individuals are the crucial steps in the Ct spread control. During three-year period we tested 2327 genitourinary samples for Ct and 945 gynaecological samples for HR HPV with normal and abnormal cervical cytological diagnosis. Subsequently all Ct positive samples were analysed by sequencing of the amplified omp1 fragments using Applied Biosystem 3130 Genetic Analyser. Genotyping and sequence mutation analysis were performed using ABI SEQSCAPE software and compared with the reference sequences of all known Ct serotypes. HR HPV positive samples with abnormal cervical cytological diagnosis were genotyped by Linear Array HPV Genotyping Test (Roche Diagnostics). The determined serotype of Ct and HPV genotypes distribution were compared with the Ct and HPV distribution pattern in other regions of the world. The association between certain HPV genotype and cervical intraepithelial neoplasia was determined as well as mixed infections with four or more HPVs. The most prevalent Ct genotype in Osijek-Baranja County was serotype E (in concordance with Sweden and Taiwan data), followed by F, K, G, D, B and J (differs from Sweden and Taiwan data). The sequence of omp1 gene showed limited variation. HPV 16 and HPV 18 were the most common HR HPV genotypes in the cervical specimens with abnormal cervical cytological diagnosis. Obtained results also determined mixed infection of four HR HPV with one or more low risk HPV. The obtained results for Ct and HPV and further analyses in this ongoing project could be useful tool for clinical and epidemiological characterization of circulating pathogens in our community.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

Napomena
Rad je kao poster prezentiran i na skupu 14th International Congress on Infectious Diseases (ICID), održanom od 09.-12.03.2010.g., Miami, Florida, SAD ; objavljen u Knjizi sažetaka.

POVEZANOST RADA