Naslov
Tumoral heterogeneity of vascular endothelial growth factor expression and angiogenesis in renal cell carcinoma

Autori
Đorđević, Gordana ; Jonjić, Nives

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11th Ljudevit Jurak International Symposium on Comparative Pathology : book of abstracts / Krušlin, Božo ; Blicza, Mladen - Zagreb : Academy of Medical of Croatia, 2000

ISBN
953-97282-2-3

Skup
Ljudevit Jurak International Symposium on Comparative Pathology (11 ; 2000)

Mjesto i datum
Zagreb, Hrvatska, 09.06.2000. - 10.06.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Sažetak
Clinical data clearly indicates a correlation between tumour neovascularisation aggressiveness of tumour growth, and metastatic spread. The vascular endotelial growth factor (VEGF) is known to represent one of the most important angiogenic factors. The present study was planned to demonstrate anfiogenesis by average microvessel density (MVD) in various types of renal cell carcinoma (RCC) regarding the nuclear grade and different histologic type, and to investigate changes in VEGF expression. Surgical speciments from 53 RCC were examined for MVD and VEGF by immunohistochemical staining, using mAb to FVIII to visualize endothelial cells, and pAb for VEGF expression. Microvessel count according to histological type was highest in mixed and the lowestn in papillary type of RCC. The rise of nuclear grade in the clear cell type, papillary type and oncocytoma was related to lower MVD. In eosinophil type it was quite opposite, while mixed type did not show variation of MVD regarding the nuclear grade. Percenatge of positive cells and staining pattern of VEGF was different between various types of tumour cells. In general, in eosinophil type of RCC, almost all cells showed diffused expression for VEGF, while in clear cell type, percenatge of positive cells varied from 20% to 100%, with the most of cells showing membrane or annular staining pattern. Furthermore, it could be observed that in clear cell type, the expression of VEGF becomes slightly more presents with higher nuclear grade. In this preliminary study we could not find a statistically significant correlation between VEGF expression and MVD. Moreover, the observation that VEGF expression is slightly higher in clear cell type of RCC with higher nuclear grade is in contrast with findings of MVD. This study needs further investigation in oreder to elucidate this heterogeneity of VEGF expression in RCC.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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