Naslov
Integrated biocatalytic hexanol oxidation in two microreactor chips connected in series

Autori
Šalić, Anita ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3rd Multistep Enzyme Catalyzed Processes Congress / - , 2014, 33-33

Skup
3rd Multistep Enzyme Catalyzed Processes Congress

Mjesto i datum
Madrid, Španjolska, 07.04.2014. - 10.04.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hexanal; microreactor; integrated system

Sažetak
Hexanal is a part of the group of easily volatile components known as "green notes". Because of its organoleptic properties hexanal has found wide application in industry. Normally it is produced by fermentation, enzyme-catalyzed reactions or by extraction from plants. The main disadvantages of these processes are low yields, formation of undesired by-products and the formation of large amounts of waste. In our previous works hexanal was produced in a microreactor using alcohol dehydrogenase (ADH) as a biocatalyst. Obtained results (Xhexanol = 80%, τ = 72 s) indicated that microreactors could be a good alternative to classical production processes. Coenzyme regeneration catalyzed by the same enzyme (ADH) that catalyzed the main reaction was performed in order to make the process more sustainable and acetaldehyde was used as a substrate. Different forms of biocatalyst were studied (suspended and immobilized ADH and permeabilized baker’s yeast cells as the source of ADH). The best results were obtained by using suspended enzyme where 100% conversion of NADH was achieved (τ = 0.8 s). Final phase of investigation was development of integrated system with two microreactor chips connected in series. One chip was used for hexanol oxidation and the second one for the simultaneous coenzyme regeneration. Regenerated coenzyme was reused by recirculation into chip where oxidation step was continuously performed for 3 days without need for addition of fresh coenzyme.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

Napomena
Ovaj rad financiran je iz sredstava Kratkoročne financijske potpore istraživanjima Sveučilišta u Zagrebu

POVEZANOST RADA