Naslov
Regeneration of coenzyme NAD+ catalyzed by NADH oxidase isolated from Lactococcus lactis in two reaction systems

Autori
Sudar, Martina ; Findrik Zvjezdana ; Vasić-Rački, Đurđa ; Clapés, Pere

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3rd Multistep Enzyme Catalyzed Processes Congress / - , 2014, 50-50

Skup
3rd Multistep Enzyme Catalyzed Processes Congress

Mjesto i datum
Madrid, Španjolska, 07.04.2014. - 10.04.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
coenzyme regeneration; NADH oxidase

Sažetak
The number of biotransformations that use nicotinamide coenzymes is constantly growing [1]. The use of these cofactors in stoichiometric amounts is too expensive and it is necessary to develop a simple and efficient cofactor recycling systems [2, 3], i.e. an in situ cofactor regeneration system. Considering the available methods for the in situ cofactor regeneration [4], a simple enzyme-coupled regeneration method, in which a second enzyme is added to the reaction system [3], was used in this work. NADH oxidase [1] only recently became commercially available, but at a very high price. Therefore, NADH oxidase was isolated from Lactococcus lactis and partially purified. The partially purified enzyme was used for NAD+ regeneration in two reactions systems. The first one is the reaction of L-methionine oxidation catalyzed by L-phenylalanine dehydrogenase. The second one is a cascade reaction of alcohol N-Cbz-3-aminopropanol oxidation and aldol addition of dihydroxyacetone to N-Cbz-3-aminopropanal in which alcohol dehydrogenase, an enzyme that requires coenzyme NAD+ for its catalytic function, catalyzes alcohol oxidation and aldolase catalyzes the second reaction.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA