Naslov
Gangliosides of human cerebrospinal fluid in various neurologic diseases

Autori
Trbojević-Čepe, Milica ; Kračun, Ivica ; Jušić, Anica ; Pavliček, Ivan

Izvornik
Journal of the neurological sciences (0022-510X) 105 (1991), 2; 192-199

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, stručni

Ključne riječi
cerebrospinal fluid; gangliosides; neurologic diseases; quantification

Sažetak
Simultaneous profile determination and quantification of human cerebrospinal fluid (CSF) gangliosides in various neurologic diseases (n = 71) was examined. Gangliosides were extracted with methanol/chloroform from clinically available amounts of CSF (4-5 ml), then separated and quantified by high-performance thin-layer chromatography (HPTLC) and direct densitometry. Based on chromatographic comparison with standards, the percentage of lipid-bound NeuAc positive fractions in 'normal' CSF samples were: GM1 (II3 NeuAc-GgOse4Cer) (3%) ; GD3 (II3 NeuAc2-Lac-Cer) (4%) ; GD1a (IV3 NeuAc, II3 NeuAc-GgOse4 Cer) (15%) ; X1 (3%) ; GD1b (II3(NeuAc)2-GgOse4 Cer) (16%) ; X2 (4%) ; GT1b (IV3 NeuAc, II3(NeuAc)2-GgOse4-Cer) (40%) ; and GQ1b (IV3(NeuAc)2, II3(NeuAc)2 - GgOse4-Cer (15%). Similarity between CSF and human cerebellar cortex, particularly in proportion of 'b' series gangliosides (GQ1b, GT1b, GD1b), could be observed. A higher proportion of GD1a ganglioside, with decreased GQ1b was found in infancy. The total ganglioside content (mean ± 2 SD) varied between 645-894 μg/l. Significant alterations of the CSF ganglioside profile, with an increase in less polar gangliosides, GM3 and GD3, correlated with the blood-brain barrier dysfunction (CSF hemorrhages, compressive syndrom), or some malignant processes (metastatic brain melanoma). A statistically significant increase in the content of total CSF gangliosides was found in the following groups of patients as compared to controls: (1) ischemic cerebrovascular accident (CVI) with good outcome (P < 0.02) ; (2) peripheral neuropathy and polyneuropathy (P < 0.001) and (3) intravertebral discopathy (P < 0.05). A significant decrease in the content of total CSF gangliosides was found in CVI group with lethal outcome (P < 0.05).

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA