Pregled bibliografske jedinice broj: 6854
Amplification of mitochondrial DNA in CGL in transformation
Amplification of mitochondrial DNA in CGL in transformation // Blood / Griffin, James (ur.).
Orlando (FL): WB Saunders, 1996. str. 234-234 (poster, međunarodna recenzija, sažetak, ostalo)
CROSBI ID: 6854 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Amplification of mitochondrial DNA in CGL in transformation
Autori
Fidler, C. ; Mills, K.I. ; Frodsham, P.M. ; Kušec, Rajko ; Gaiger, Alex ; Gale, R.E. ; Linch, D.C. ; Littlewood, T.J. ; Moss, P.A.H. ; Boultwood, J. ; Wainscoat, J.S.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo
Izvornik
Blood
/ Griffin, James - Orlando (FL) : WB Saunders, 1996, 234-234
Skup
American Society of Hematology Annual Meeting
Mjesto i datum
Orlando (FL), Sjedinjene Američke Države, 06.12.1996. - 10.12.1996
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
mitochondrial DNA; leukaemia
Sažetak
There is evidence to suggest that alterations in mitochondrial DNA (mtDNA) may contribute to the tumorigenic phenotype of human malignancy. We have demonstrated that mtDNA is often remarkably amplified (2fold) in the blast cells of human acute myeloid leukemia (AML).1 We used gene dosage experiments to quantify the amount of mtDNA relative to human nuclear DNA. DNA extracted from the peripheral blood leukocytes or bone marrow of healthy individuals or patients was simultaneously hybridized with a probe for the mitochondrial genome and a control probe for the renin gene on human chromosome 1. Comparative densitometric ratios of approximately 1 were obtained between the two signals in 20 normal control peripheral blood samples and the bone marrow biopsies from 4 normal individuals. We sought to discover whether amplification of mtDNA occured in chronic granulocytic leukemia (CGL). Comparative densitometric ratios essentially the same as those observed in the peripheral blood samples from healthy controls i.e. approximately one were observed in 18 CGL samples in the chronic phase of the disease. The DNA samples of 9 cases of CGL were examined in both the chronic phase and in transformation to AML. In 8/9 cases amplification of mtDNA (3fold) was found in samples from the cases in transformation. We conclude that amplification of mtDNA is an invariable feature of acute myeloid leukemia and that it may be a useful marker for detecting transformation of CGL.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
